Jennifer Kohler completed her undergraduate degree in Chemistry at Bryn Mawr College. Her Ph.D. studies, focused on the kinetics of protein-DNA interactions, were conducted in the laboratory of Prof. Alanna Schepartz, in the Chemistry Department at Yale University. From 2000-2004, Jennifer was an American Cancer Society postdoctoral fellow with Prof. Carolyn Bertozzi at the University of California, Berkeley. Research in the Kohler lab focuses on understanding the roles of glycoconjugates in a variety of biological systems.
Glycosylation is the elephant in the room of biomedical research. Estimates suggest that more than 50% of eukaryotic proteins are glycosylated, and new forms of protein glycosylation are still being discovered. Glycosylation is also a common feature of lipids, with at least 200 distinct glycolipid structures known in eukaryotes. Unconjugated polysaccharide chains are also abundant and diverse in structure. In fact, about 2% of human genes are involved in carbohydrate metabolism and glycosylation. Individual differences in glycosylation may underlie much of human variation. Clearly, evolution has favored an emphasis on glycosylation; however, the modern research environment is less conducive to focusing on carbohydrate-containing molecules.
Unfortunately, many of the biochemical and analytical techniques that are used to study protein-protein interactions are poorly suited to the study of glycosylated molecules. First, glycan-mediated interactions are typically low affinity and do not survive the rigorous purification steps often used to identify binding partners. Second, protein-centric methods do not take into account that fact that glycosylated proteins typically exist as a mixture of glycoforms, each of which may have unique binding properties and activities. Finally, in many techniques (yeast two-hybrid, heterologous expression systems) the critical glycans are either absent on altered.
My research group at UT Southwestern is committed to developing and implementing new tools that are optimized for the study of glycosylated molecules. In particular, we invested significant effort in the development of photocrosslinking sugar analogs that can be metabolically incorporated into cellular glycoconjugates and used to covalently crosslink glycan-mediated interactions. These tools can now be deployed to study and identify transient glycan-mediated interactions. Our current research efforts are focused in two broad areas: (1) sialic acid-containing glycoconjugates (sialosides); and (2) O-GlcNAc-modified proteins. We are currently using photocrosslinking sialic acid analogs to study the interactions of sialic acid-interacting proteins, particularly those involved in infectious disease and in cancer metastasis. We are using photocrosslinking GlcNAc to investigate the interactions of nucleoporins and other O-GlcNAc-modified proteins. In addition to our photocrosslinking studies, we have developed a new two-hybrid technique that can be used to interrogate protein-protein interactions in the Golgi and eukaryotic cells.
- Graduate School
- Yale University , Chemistry
- Graduate School
- Bryn Mawr College (1994), Chemistry
- chemical biology
- membrane proteins
- Modified GM3 gangliosides produced by metabolic oligosaccharide engineering.
- Whitman CM, Yang F, Kohler JJ Bioorganic & medicinal chemistry letters 2011 May 5006-10
- Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway.
- Boyce M, Carrico IS, Ganguli AS, Yu SH, Hangauer MJ, Hubbard SC, Kohler JJ, Bertozzi CR Proc. Natl. Acad. Sci. U.S.A. 2011 Feb 108 8 3141-6
- Metabolically incorporated photocrosslinking sialic acid covalently captures a ganglioside-protein complex.
- Bond MR, Whitman CM, Kohler JJ Mol Biosyst 2010 Oct 6 10 1796-9
- Post-translational modifications: A shift for the O-GlcNAc paradigm.
- Kohler JJ Nat. Chem. Biol. 2010 Sep 6 9 634-5
- Regulation of intracellular signaling by extracellular glycan remodeling.
- Parker RB, Kohler JJ ACS Chem. Biol. 2010 Jan 5 1 35-46
- Metabolic labeling of glycoconjugates with photocrosslinking sugars.
- Yu SH, Bond MR, Whitman CM, Kohler JJ Meth. Enzymol. 2010 478 541-62
- A two-hybrid assay to study protein interactions within the secretory pathway.
- Dube DH, Li B, Greenblatt EJ, Nimer S, Raymond AK, Kohler JJ PLoS ONE 2010 5 12 e15648
- Effects of N-glycosylation on the activity and localization of GlcNAc-6-sulfotransferase 1.
- Desko MM, Gross DA, Kohler JJ Glycobiology 2009 Oct 19 10 1068-77
- Aniline: a catalyst for sialic acid detection.
- Kohler JJ Chembiochem 2009 Sep 10 13 2147-50
- Association of beta-1,3-N-acetylglucosaminyltransferase 1 and beta-1,4-galactosyltransferase 1, trans-Golgi enzymes involved in coupled poly-N-acetyllactosamine synthesis.
- Lee PL, Kohler JJ, Pfeffer SR Glycobiology 2009 Jun 19 6 655-64
Mass Spectrometry of Glycoproteins: Methods and Protocols
Jennifer J. Kohler & Steven M. Patrie (Ed.) (2012). Humana Press
Honors & Awards
- Research Fellowship
Alfred P. Sloan Foundation (2009)
- Basil O’Connor Starter Scholar Research Award
March of Dimes (2007)
- CAREER Award
National Science Foundation (2007)
- New Faculty Award
Camille and Henry Dreyfus Foundation (2005)
- Society for Glycobiology (2007)
- American Chemical Society (1995)