Education

Undergraduate
Univ of California-Berkeley (1997), Molecular & Cell Biology
Undergraduate
Univ of California-Berkeley (1997), Bioengineering
Graduate School
Stanford University (2002), Biophysics

Research Interest

  • How signaling by membrane cholesterol controls cell growth, maintains lipid homeostasis, and protects us from microbial infection

Publications

Featured Publications LegendFeatured Publications

A cholesterol-binding bacterial toxin provides a strategy for identifying a specific Scap inhibitor that blocks lipid synthesis in animal cells.
Xu S, Smothers JC, Rye D, Endapally S, Chen H, Li S, Liang G, Kinnebrew M, Rohatgi R, Posner BA, Radhakrishnan A, Proc Natl Acad Sci U S A 2024 Feb 121 7 e2318024121
DGAT2 inhibition blocks SREBP-1 cleavage and improves hepatic steatosis by increasing phosphatidylethanolamine in the ER.
Rong S, Xia M, Vale G, Wang S, Kim CW, Li S, McDonald JG, Radhakrishnan A, Horton JD, Cell Metab 2024 Feb
A concerted mechanism involving ACAT and SREBPs by which oxysterols deplete accessible cholesterol to restrict microbial infection.
Heisler DB, Johnson KA, Ma DH, Ohlson MB, Zhang L, Tran M, Corley CD, Abrams ME, McDonald JG, Schoggins JW, Alto NM, Radhakrishnan A, Elife 2023 Jan 12
Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling.
Kinnebrew M, Johnson KA, Radhakrishnan A, Rohatgi R, Methods Mol Biol 2022 2374 73-87
Patched 1 reduces the accessibility of cholesterol in the outer leaflet of membranes.
Kinnebrew M, Luchetti G, Sircar R, Frigui S, Viti LV, Naito T, Beckert F, Saheki Y, Siebold C, Radhakrishnan A, Rohatgi R, Elife 2021 Oct 10
Scap structures highlight key role for rotation of intertwined luminal loops in cholesterol sensing.
Kober DL, Radhakrishnan A, Goldstein JL, Brown MS, Clark LD, Bai XC, Rosenbaum DM, Cell 2021 Jun
The use of anthrolysin O and ostreolysin A to study cholesterol in cell membranes.
Johnson KA, Radhakrishnan A, Methods Enzymol 2021 649 543-566
Cholesterol access in cellular membranes controls Hedgehog signaling.
Radhakrishnan A, Rohatgi R, Siebold C, Nat Chem Biol 2020 Dec 16 12 1303-1313
Identification of a degradation signal at the carboxy terminus of SREBP2: A new role for this domain in cholesterol homeostasis.
Kober DL, Xu S, Li S, Bajaj B, Liang G, Rosenbaum DM, Radhakrishnan A, Proc Natl Acad Sci U S A 2020 Oct
Accessible cholesterol is localized in bacterial plasma membrane protrusions.
Abrams ME, Johnson KA, Radhakrishnan A, Alto NM, J. Lipid Res. 2020 Jul