Dr. Rosen is the Chair of the Department of Biophysics at UT Southwestern Medical Center and an Investigator of the Howard Hughes Medical Institute. Dr. Rosen received undergraduate degrees in chemistry and in chemical engineering from the University of Michigan in 1987. He then spent a year in Alan Battersby’s lab in the Department of Chemistry at the University of Cambridge as a Winston Churchill Foundation Scholar. He received his Ph.D. in Chemistry from Harvard University in 1993 under the direction of Stuart Schreiber, where he studied the structure and function of the FK506 binding protein, FKBP12. He was a Damon Runyon-Walter Winchell post-doctoral fellow in the laboratories of Tony Pawson and Lewis Kay at the University of Toronto, where he studied regulation of the signaling adaptor protein, Crk, and developed methods of selective methyl group labeling of proteins for NMR spectroscopy. Dr. Rosen started his independent laboratory in 1996 in the Cellular Biochemistry and Biophysics Program at the Memorial Sloan-Kettering Cancer Center in New York City, and moved to UTSW in 2001.
The Rosen lab seeks to understand the formation, regulation and functions of enigmatic, cellular compartments termed biomolecular condensates. These evolutionarily conserved structures concentrate diverse but specific groups of molecules without a surrounding membrane. Condensates appear to form through the physical process of liquid-liquid phase separation. Using a range of techniques, including biochemical reconstitution and in vitro and cellular microsopies, we investigate phase separation in both engineered and natural condensates. The former, in their simplified nature, enable precise isolation of key molecular parameters governing condensate behaviors, revealing general principles. The latter allow demonstration of these principles in more complex natural biochemical and cellular systems. Ultimately, we seek to understand cell organization on scales spanning nanometers to microns.
The Rosen lab website can be found at: www.utsouthwestern.edu/labs/rosen/
- University of Michigan-Ann Arb (1987), Chemical Engineering
- University of Michigan-Ann Arb (1987), Chemistry
- Graduate School
- Harvard University (1993), Organic Chemistry
- Biological phase separation
- cell biology of the actin cytoskeleton
- polymer physics/chemistry
- structural biology
- Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization.
- Tskvitaria-Fuller I, Seth A, Mistry N, Gu H, Rosen MK, Wülfing C J. Immunol. 2006 Aug 177 3 1708-20
- Protein-tyrosine kinase and GTPase signals cooperate to phosphorylate and activate Wiskott-Aldrich syndrome protein (WASP)/neuronal WASP.
- Torres E, Rosen MK J. Biol. Chem. 2006 Feb 281 6 3513-20
- Biochemical properties and inhibitors of (N-)WASP.
- Leung DW, Morgan DM, Rosen MK Meth. Enzymol. 2006 406 281-96
- Acidic region tyrosines provide access points for allosteric activation of the autoinhibited Vav1 Dbl homology domain.
- Amarasinghe GK, Rosen MK Biochemistry 2005 Nov 44 46 15257-68
- NMR analyses of the activation of the Arp2/3 complex by neuronal Wiskott-Aldrich syndrome protein.
- Kreishman-Deitrick M, Goley ED, Burdine L, Denison C, Egile C, Li R, Murali N, Kodadek TJ, Welch MD, Rosen MK Biochemistry 2005 Nov 44 46 15247-56
- A novel mechanism of actin filament processive capping by formin: solution of the rotation paradox.
- Shemesh T, Otomo T, Rosen MK, Bershadsky AD, Kozlov MM J. Cell Biol. 2005 Sep 170 6 889-93
- [Structure and function of Formin homology 2 domain].
- Otomo T, Rosen MK Tanpakushitsu Kakusan Koso 2005 Aug 50 9 1088-93
- Structural basis of Rho GTPase-mediated activation of the formin mDia1.
- Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK Mol. Cell 2005 Apr 18 3 273-81
- The nucleotide switch in Cdc42 modulates coupling between the GTPase-binding and allosteric equilibria of Wiskott-Aldrich syndrome protein.
- Leung DW, Rosen MK Proc. Natl. Acad. Sci. U.S.A. 2005 Apr 102 16 5685-90
- Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain.
- Otomo T, Tomchick DR, Otomo C, Panchal SC, Machius M, Rosen MK Nature 2005 Feb 433 7025 488-94
Honors & Awards
- Allen Distinguished Investigator, a program funded through The Paul G. Allen Frontiers Group
- Emil Kaiser Award, Protein Society
- Mar Nell and F. Andrew Bell Distinguished Chair in Biochemistry
- Carolyn R. Bacon Professorship in Medical Science and Education
- Edith & Peter O’Donnell Award from the Texas Academy of Medicine, Engineering and Science
- Howard Hughes Medical Institute
- Boyer Award, Memorial Sloan-Kettering Cancer Center
- Sidney Kimmel Scholar Award
- Beckman Young Investigator
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- Winston Churchill Scholar