Dr. Rosen is the Chair of the Department of Biophysics at UT Southwestern Medical Center and an Investigator of the Howard Hughes Medical Institute. Dr. Rosen received undergraduate degrees in chemistry and in chemical engineering from the University of Michigan in 1987. He then spent a year in Alan Battersby’s lab in the Department of Chemistry at the University of Cambridge as a Winston Churchill Foundation Scholar. He received his Ph.D. in Chemistry from Harvard University in 1993 under the direction of Stuart Schreiber, where he studied the structure and function of the FK506 binding protein, FKBP12. He was a Damon Runyon-Walter Winchell post-doctoral fellow in the laboratories of Tony Pawson and Lewis Kay at the University of Toronto, where he studied regulation of the signaling adaptor protein, Crk, and developed methods of selective methyl group labeling of proteins for NMR spectroscopy. Dr. Rosen started his independent laboratory in 1996 in the Cellular Biochemistry and Biophysics Program at the Memorial Sloan-Kettering Cancer Center in New York City, and moved to UTSW in 2001.
The Rosen lab seeks to understand the formation, regulation and functions of enigmatic, cellular compartments termed biomolecular condensates. These evolutionarily conserved structures concentrate diverse but specific groups of molecules without a surrounding membrane. Condensates appear to form through the physical process of liquid-liquid phase separation. Using a range of techniques, including biochemical reconstitution and in vitro and cellular microsopies, we investigate phase separation in both engineered and natural condensates. The former, in their simplified nature, enable precise isolation of key molecular parameters governing condensate behaviors, revealing general principles. The latter allow demonstration of these principles in more complex natural biochemical and cellular systems. Ultimately, we seek to understand cell organization on scales spanning nanometers to microns.
The Rosen lab website can be found at: www.utsouthwestern.edu/labs/rosen/
- University of Michigan-Ann Arb (1987), Chemistry
- University of Michigan-Ann Arb (1987), Chemical Engineering
- Graduate School
- Harvard University (1993), Organic Chemistry
- Biological phase separation
- cell biology of the actin cytoskeleton
- polymer physics/chemistry
- structural biology
- Purification of Arp2/3 Complex from Saccharomyces cerevisiae.
- Doolittle LK, Rosen MK, Padrick SB Methods Mol. Biol. 2013 1046 251-71
- Purification of native arp2/3 complex from bovine thymus.
- Doolittle LK, Rosen MK, Padrick SB Methods Mol. Biol. 2013 1046 231-50
- Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation.
- Smith BA, Padrick SB, Doolittle LK, Daugherty-Clarke K, Corrêa IR, Xu MQ, Goode BL, Rosen MK, Gelles J Elife 2013 2 e01008
- Enlightening molecular mechanisms through study of protein interactions.
- Rizo J, Rosen MK, Gardner KH J Mol Cell Biol 2012 Oct 4 5 270-83
- Multiple repeat elements within the FAM21 tail link the WASH actin regulatory complex to the retromer.
- Jia D, Gomez TS, Billadeau DD, Rosen MK Mol. Biol. Cell 2012 Jun 23 12 2352-61
- Mechanism of actin filament nucleation by the bacterial effector VopL.
- Yu B, Cheng HC, Brautigam CA, Tomchick DR, Rosen MK Nat. Struct. Mol. Biol. 2011 Sep 18 9
- The feasibility of parameterizing four-state equilibria using relaxation dispersion measurements.
- Li P, Martins IR, Rosen MK J. Biomol. NMR 2011 Sep 51 1-2 57-70
- Determination of protein complex stoichiometry through multisignal sedimentation velocity experiments.
- Padrick SB, Deka RK, Chuang JL, Wynn RM, Chuang DT, Norgard MV, Rosen MK, Brautigam CA Anal. Biochem. 2010 Dec 407 1 89-103
- Structure and control of the actin regulatory WAVE complex.
- Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK Nature 2010 Nov 468 7323 533-8
- Rationally improving LOV domain-based photoswitches.
- Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR Nat. Methods 2010 Aug 7 8 623-6
Honors & Awards
- Allen Distinguished Investigator, a program funded through The Paul G. Allen Frontiers Group
- Emil Kaiser Award, Protein Society
- Mar Nell and F. Andrew Bell Distinguished Chair in Biochemistry
- Carolyn R. Bacon Professorship in Medical Science and Education
- Edith & Peter O’Donnell Award from the Texas Academy of Medicine, Engineering and Science
- Howard Hughes Medical Institute
- Boyer Award, Memorial Sloan-Kettering Cancer Center
- Sidney Kimmel Scholar Award
- Beckman Young Investigator
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- Winston Churchill Scholar