Research in my laboratory investigates chemical, stress and nutritional signaling at the interface amongst the mammalian host, beneficial microbiota and invading pathogens. We devise a multi-disciplinary research program utilizing genetic, biochemical, chemical and structural approaches to investigate fundamental biological questions. Our research over the years has focused on:
1) Bacterial cells sense several mammalian neurotransmitters as a means to gage the physiological and immune state of the host, leading to rewiring and reprogramming of bacterial transcription towards host and niche adaptation. We have also identified several bacterial adrenergic, endocannabinoid and serotonergic receptors.
2) We have shown that these bacterial neurotransmitter receptors also sense bacterial signals, such as the novel pirazynone family of AI-3 autoinducers, and indole, which in turn influence mammalian cell signaling, linking inter-kingdom chemical communication at the biochemical level. These studies highlight the co-evolution and the fundamental relationship between mammals and microbes, and that these signaling systems fully integrate bacterial and mammalian cell-behavior.
3) We also reported that invading pathogens can hijack these inter-kingdom signaling systems to promote virulence expression. We translated these basic science concepts into strategies to develop a novel approach to anti-virulence therapies.
4) Our work also explored how different chemical signaling systems shape bacterial/host relationships towards commensalism or pathogenesis. This work shows that different chemical relationships define the nature of host/bacterial associations.
5) We have also shown that enteric pathogens exploit nutritional cues (Carbon, aminoacids and/or nitrogen sources) made available by the gut microbiota as signals to coordinate virulence regulation and modify the pathogen’s metabolism allowing for efficient host colonization.
6) Moreover the cross-signaling with neurotransmitters, which is a key event I the gut-brain-axis, can also lead to new insights into drug addiction, and repurposing of agonists and antagonists of the adrenergic, serotonergic and endocannabinoid systems as potential novel anti-bacterial therapies.
- Graduate School
- Unlisted - International Colle
- Graduate School
- Campinas State University (1995), Molecular Biology
- addiction and the microbiome
- Bacterial pathogenesis
- Bacterial virulence gene expression
- E. coli O157:H7
- gut brain axis
- Inter-kingdom signaling
- pathogen-microbiota-host interactions
- Quorum sensing
- Take Your Pick: Vitamins and Microbiota Facilitate Pathogen Clearance.
- Sperandio V Cell Host Microbe 2017 Feb 21 2 130-131
- Genetic and mechanistic analyses of the periplasmic domain of the enterohemorrhagic E. coli (EHEC) QseC histidine sensor kinase.
- Parker CT, Russell R, Njoroge JW, Jimenez AG, Taussig R, Sperandio V J. Bacteriol. 2017 Jan
- Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites.
- Yamamoto D, Hernandes RT, Liberatore AM, Abe CM, Souza RB, Romão FT, Sperandio V, Koh IH, Gomes TA PLoS ONE 2017 12 2 e0171385
- Catabolite and Oxygen Regulation of Enterohemorrhagic Escherichia coli Virulence.
- Carlson-Banning KM, Sperandio V MBio 2016 Nov 7 6
- Interactions between the microbiota and pathogenic bacteria in the gut.
- Bäumler AJ, Sperandio V Nature 2016 Jul 535 7610 85-93
- In vivo influence of in vitro up-regulated genes in the virulence of an APEC strain associated with swollen head syndrome.
- de Paiva JB, da Silva LP, Casas MR, Conceição RA, Nakazato G, de Pace F, Sperandio V, da Silveira WD Avian Pathol. 2016 Feb 45 1 94-105
- Bacterial Adrenergic Sensors Regulate Virulence of Enteric Pathogens in the Gut.
- Moreira CG, Russell R, Mishra AA, Narayanan S, Ritchie JM, Waldor MK, Curtis MM, Winter SE, Weinshenker D, Sperandio V MBio 2016 7 3
- The Epinephrine/Norepinephrine /Autoinducer-3 Interkingdom Signaling System in Escherichia coli O157:H7.
- Moreira CG, Sperandio V Adv. Exp. Med. Biol. 2016 874 247-61
- What a Dinner Party! Mechanisms and Functions of Interkingdom Signaling in Host-Pathogen Associations.
- Kendall MM, Sperandio V MBio 2016 7 2
- Frenemies: Signaling and Nutritional Integration in Pathogen-Microbiota-Host Interactions.
- Cameron EA, Sperandio V Cell Host Microbe 2015 Sep 18 3 275-84
The epinephrine/norepinephrine/autoinducer-3 inter-kingdom signaling system in Escherichia coli O157:H7. In Microbial endocrinology
Moreira, C.G., and Sperandio, V. (2010). New York, Springer
Regulation in Response to Host-derived Signaling Molecules. In Regulation of Bacterial Virulence
Gruber, C, and Sperandio, V. (2013). Washington DC, ASM Press
Enterohemorrhagic and Shiga toxin producing Escherichia coli.
Sperandio V, Hovde, C.J. (Ed.) (2014). Washington DC, ASM Press
Attaching and effacing intestinal histopathology and the locus of enterocyte effacement.. In Escherichia coli O157:H7 and other Shiga-toxin producing E. coli strains.
Kaper, J.B., Elliott, S.J., Sperandio, V., Perna, N.T., Mayhew, G.F., and Blattner, F.R. (1998). Washington DC, ASM Press
LuxS dependent regulation of E. coli virulence. In Bacterial cell-to-cell communication role in virulence and pathogenesis
Clarke, M.B., and Sperandio, V. (2006). Cambridge, U.K., Cambridge University Press
Quorum Sensing in E. coli and Salmonella. In E. coli and Salmonella book
Kendall, M., and Sperandio, V. (2014). Washington DC, ASM Press
The interplay between the microbiota and EHEC. In Enterohemorrhagic Escherichia coli.
Pifer, R. and Sperandio V. (2014). Washington DC, ASM Press
The Way Forward. In Sperandio. In Enterohemorrhagic Escherichia coli
Sperandio V. 2014 (2014). Washington DC, ASM Press
Interference with bacterial cell-to-cell chemical signaling in development of new anti-infectives. In Antibiotics, Targets, Maechanisms, and Resistance
Njoroge, J.W. and Sperandio, V (2013). Weinheim, Germany, Wiley-VCH
Enteric Pathogens Exploit the Microbiota-generated Nutritional Environment of the gut. In Metabolism and Bacterial Pathogenesis.
Pacheco, A.R., and Sperandio V. (2015). Washington DC, ASM Press
Quorum sensing and the gut microbiome. In Quorum Sensing: molecular mechanism and biotechnological application
Jimenez, A.G. and Sperandio V (2019). New York, Elsevier
Honors & Awards
- NIH Merit award
grant Meirt award (2017-2027)
- ASM Eli-Lilly and Company-Elanco research award
Oldest and most pretigious ASM research award (2015-2015)
- GSK Discovery Fast Track challenge program
drug discovery award (2014-2015)
- Fellow American Academy of Microbiology
Fellow of the AAM (2013)
- Kavli Fellow
Kavli Frontiers of Science National Academies of Science (2007)
- Burroughs Wellcome Fund
Investigator in Pathogenesis of Infectious Diseases (2006)
- Ellison Foundation Award
New Scholar of Infectious diseases (2004)
- Dam Charitable Foundation
Travel Grant (2000)
- Pew Charitable Foundation
Pew Latin american fellow (1997)
- Vanessa Sperandio (2001-2020)