Chad Brautigam, Ph.D.

• Analytical Ultracentrifugation
• Isothermal Titration Calorimetry
• Protein-protein interactions
• Structural Biology/X-ray Crystallography
• Structural Elucidation of Enzymatic Mechanisms

 Featured Publications

The Cytosolic DNA Sensor cGAS Forms an Oligomeric Complex with DNA and Undergoes Switch-like Conformational Changes in the Activation Loop.

Zhang X, Wu J, Du F, Xu H, Sun L, Chen Z, Brautigam CA, Zhang X, Chen ZJ Cell Rep 2014 Feb 6 3 421-30

Sequence, biophysical, and structural analyses of the PstS lipoprotein (BB0215) from Borrelia burgdorferi reveal a likely binding component of an ABC-type phosphate transporter.

Brautigam CA, Ouyang Z, Deka RK, Norgard MV Protein Sci. 2014 Feb 23 2 200-12

Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation.

Zhao H, Brautigam CA, Ghirlando R, Schuck P Curr Protoc Protein Sci 2013 Feb Chapter 20 Unit20.12

Evidence for an ABC-Type Riboflavin Transporter System in Pathogenic Spirochetes.

Deka RK, Brautigam CA, Biddy BA, Liu WZ, Norgard MV MBio 2013 4 1

Structural insight into interactions between dihydrolipoamide dehydrogenase (E3) and E3 binding protein of human pyruvate dehydrogenase complex.

Brautigam, C.A., Wynn, R.M., Chuang, J.C., Machius, M., Tomchick, D.R., and Chuang, D.T. Structure 2006 14 611-621

Crystal structure of the photolyase-like domain of Cryptochome 1 from Arabidopsis thaliana.

Brautigam, C.A., Smith, B.S., Ma, Z., Palnitkar, M., Tomchick, D.R., Machius, M., and Deisenhofer, J. Proc. Natl. Acad. Sci. (USA) 2004 101 12142-12147

Haemophilus ducreyi Hfq contributes to virulence gene regulation as cells enter stationary phase.

Gangaiah D, Labandeira-Rey M, Zhang X, Fortney KR, Ellinger S, Zwickl B, Baker B, Liu Y, Janowicz DM, Katz BP, Brautigam CA, Munson RS, Hansen EJ, Spinola SM MBio 2014 5 1 e01081-13

Improving the Thermal, Radial and Temporal Accuracy of the Analytical Ultracentrifuge through External References.

Ghirlando R, Balbo A, Piszczek G, Brown PH, Lewis MS, Brautigam CA, Schuck P, Zhao H Anal. Biochem. 2013 May

Trypanosoma brucei S-Adenosylmethionine Decarboxylase N Terminus Is Essential for Allosteric Activation by the Regulatory Subunit Prozyme.

Velez N, Brautigam CA, Phillips MA J. Biol. Chem. 2013 Feb 288 7 5232-40

Multi-signal sedimentation velocity analysis with mass conservation for determining the stoichiometry of protein complexes.

Brautigam CA, Padrick SB, Schuck P PLoS ONE 2013 8 5 e62694

• Magna cum laude
  University of Colorado at Boulder (1991)
• Phi Beta Kappa
  University of Colorado at Boulder (1991)
• Williams Family Foundation Scholarship
  High School (1987)