Glen Liszczak is an Assistant Professor in the Department of Biochemistry at the University of Texas Southwestern Medical Center. He received his B.S. in Chemistry and Biology from Ramapo College of New Jersey and his Ph.D. in Chemistry from the University of Pennsylvania at the Wistar Institute under the supervision of Dr. Ronen Marmorstein. As a graduate student, Glen used X-ray crystallography and other biochemical and biophysical tools to determine the molecular basis for catalysis and substrate specificity exhibited by protein-modifying enzymes. Such enzymes included lysine and amino-terminal acetyltransferases that represent potential therapeutic targets in a variety of diseases.
In 2013, Glen joined the laboratory of Professor Tom Muir as a postdoctoral fellow at Princeton University where he employed a wide array of chemical biology techniques to delineate epigenetic mechanisms and their misregulation in cancer. During his postdoctoral studies, Glen combined protein semi-synthesis with high-throughput biochemistry and cellular biology to study proteins that interact with the genome and regulate gene expression. These efforts led to the discovery of regulatory mechanisms that modulate ADP-ribosylation of chromatin by PARP enzymes during the DNA damage response. He also developed methods to deliver synthetic entities to specific genomic loci in live cells by using bioorthogonal reactive handles to append small molecules and modified peptides to a programmable DNA binding protein (nuclease-deficient Cas9).
Throughout his career Glen has been the recipient of numerous awards for his research, including the Dr. Monica H.M. Shander Memorial Fellowship, a Ruth L. Kirschstein National Research Service Award, a Cancer Prevention Research Institute of Texas (CPRIT) Recruitment of First-Time, Tenure-Track Faculty Member Award, and a UT System Rising STARs Award. In 2018, Glen joined the faculty at UTSW as an Endowed Scholar. His lab integrates chemical and biological approaches to study nuclear protein post-translational modifications and their effect on transcription regulation and genome integrity. Ultimately, his group seeks to understand how aberrant protein modification activities contribute to genetic diseases such as cancer.
- Cancer biology
- Chemical biology and protein semi-synthesis
- Genome integrity
- Protein post-translational modifications
- Transcription regulation and epigenetics
- Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
- Liszczak G, Diehl KL, Dann GP, Muir TW Nat. Chem. Biol. 2018 Sep 14 9 837-840
- ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference.
- Dann GP, Liszczak GP, Bagert JD, Müller MM, Nguyen UTT, Wojcik F, Brown ZZ, Bos J, Panchenko T, Pihl R, Pollock SB, Diehl KL, Allis CD, Muir TW Nature 2017 08 548 7669 607-611
- Genomic targeting of epigenetic probes using a chemically tailored Cas9 system.
- Liszczak GP, Brown ZZ, Kim SH, Oslund RC, David Y, Muir TW Proc. Natl. Acad. Sci. U.S.A. 2017 Jan 114 4 681-686
- Molecular Basis for Cohesin Acetylation by Establishment of Sister Chromatid Cohesion N-Acetyltransferase ESCO1.
- Rivera-Colón Y, Maguire A, Liszczak GP, Olia AS, Marmorstein R J. Biol. Chem. 2016 Dec 291 51 26468-26477
- A two-state activation mechanism controls the histone methyltransferase Suv39h1.
- Müller MM, Fierz B, Bittova L, Liszczak G, Muir TW Nat. Chem. Biol. 2016 Mar 12 3 188-93
- Biochemical and cellular analysis of Ogden syndrome reveals downstream Nt-acetylation defects.
- Myklebust LM, Van Damme P, Støve SI, Dörfel MJ, Abboud A, Kalvik TV, Grauffel C, Jonckheere V, Wu Y, Swensen J, Kaasa H, Liszczak G, Marmorstein R, Reuter N, Lyon GJ, Gevaert K, Arnesen T Hum. Mol. Genet. 2015 Apr 24 7 1956-76
- The molecular basis for histone H4- and H2A-specific amino-terminal acetylation by NatD.
- Magin RS, Liszczak GP, Marmorstein R Structure 2015 Feb 23 2 332-41
- Implications for the evolution of eukaryotic amino-terminal acetyltransferase (NAT) enzymes from the structure of an archaeal ortholog.
- Liszczak G, Marmorstein R Proc. Natl. Acad. Sci. U.S.A. 2013 Sep 110 36 14652-7
- Molecular basis for N-terminal acetylation by the heterodimeric NatA complex.
- Liszczak G, Goldberg JM, Foyn H, Petersson EJ, Arnesen T, Marmorstein R Nat. Struct. Mol. Biol. 2013 Sep 20 9 1098-105
- Specificity and versatility of substrate binding sites in four catalytic domains of human N-terminal acetyltransferases.
- Grauffel C, Abboud A, Liszczak G, Marmorstein R, Arnesen T, Reuter N PLoS ONE 2012 7 12 e52642
Honors & Awards
- Cancer Prevention and Research Institute of Texas (CPRIT) Scholar (Recruitment of First-Time, Tenure-Track Faculty Members)
- UT Southwestern Endowed Scholar
- UT System Rising STARs Award
- NIH Ruth L. Kirschstein National Research Service Award
- Dr. Monica H.M. Shander Memorial Fellowship
- NIH Chemistry-Biology Interface Training Fellowship
- Princeton University (2013-2018)
- University of Pennsylvania (2008-2013)
- Ramapo College of New Jersey (2004-2008)