Huiming Lu, Ph.D.

Undergraduate

Zhejiang University (2004), Agriculture

Graduate School

Zhejiang University (2009), Biophysics

• DNA double-strand breaks (DSBs) induce genome instability, trigger cellular senescence and cell death, resulting in diseases. I am pursuing the molecular mechanisms of DSB repair and their relevance to unhealthy aging and tumorigenesis. I am also seeking potential clinical applications by targeting DSB repair pathways.

Featured Publications

DNA-PKcs-dependent phosphorylation of RECQL4 promotes NHEJ by stabilizing the NHEJ machinery at DNA double-strand breaks.

Lu H, Guan J, Wang SY, Li GM, Bohr VA, Davis AJ, Nucleic Acids Res 2022 May

DNA-PKcs promotes chromatin decondensation to facilitate initiation of the DNA damage response.

Lu H, Saha J, Beckmann PJ, Hendrickson EA, Davis AJ, Nucleic Acids Res. 2019 Aug

Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair.

Lu H, Shamanna RA, de Freitas JK, Okur M, Khadka P, Kulikowicz T, Holland PP, Tian J, Croteau DL, Davis AJ, Bohr VA Nat Commun 2017 Dec 8 1 2039

RECQL4 Promotes DNA End Resection in Repair of DNA Double-Strand Breaks.

Lu H, Shamanna RA, Keijzers G, Anand R, Rasmussen LJ, Cejka P, Croteau DL, Bohr VA, Cell Rep 2016 06 16 1 161-173

Ablating putative Ku70 phosphorylation sites results in defective DNA damage repair and spontaneous induction of hepatocellular carcinoma.

Saha J, Bae J, Wang SY, Lu H, Chappell LJ, Gopal P, Davis AJ, Nucleic Acids Res 2021 Aug

Human RecQ Helicases in DNA Double-Strand Break Repair.

Lu H, Davis AJ, Front Cell Dev Biol 2021 9 640755

MLH1 Deficiency-Triggered DNA Hyperexcision by Exonuclease 1 Activates the cGAS-STING Pathway.

Guan J, Lu C, Jin Q, Lu H, Chen X, Tian L, Zhang Y, Ortega J, Zhang J, Siteni S, Chen M, Gu L, Shay JW, Davis AJ, Chen ZJ, Fu YX, Li GM, Cancer Cell 2020 Dec

WRN regulates pathway choice between classical and alternative non-homologous end joining.

Shamanna RA, Lu H, de Freitas JK, Tian J, Croteau DL, Bohr VA Nat Commun 2016 Dec 7 13785

NAD(+) Replenishment Improves Lifespan and Healthspan in Ataxia Telangiectasia Models via Mitophagy and DNA Repair.

Fang EF, Kassahun H, Croteau DL, Scheibye-Knudsen M, Marosi K, Lu H, Shamanna RA, Kalyanasundaram S, Bollineni RC, Wilson MA, Iser WB, Wollman BN, Morevati M, Li J, Kerr JS, Lu Q, Waltz TB, Tian J, Sinclair DA, Mattson MP, Nilsen H, Bohr VA Cell Metab. 2016 Oct 24 4 566-581

RECQL4 helicase has oncogenic potential in sporadic breast cancers.

Arora A, Agarwal D, Abdel-Fatah TM, Lu H, Croteau DL, Moseley P, Aleskandarany MA, Green AR, Ball G, Rakha EA, Chan SY, Ellis IO, Wang LL, Zhao Y, Balajee AS, Bohr VA, Madhusudan S, J Pathol 2016 Mar 238 4 495-501

• American Association for Cancer Research (2021)
• American Society for Biochemistry and Molecular Biology (2021)
• Radiation Research Society (2018)