2011-2017 Post-doc, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
2005-2011 Ph.D., Molecular and Cell Biology, University of California, Berkeley
2001-2005 Bachelor of Science, Biology, University of Texas at Austin
Research Overview: Eggs can survive in a dormant state for many years. They then dramatically reawaken upon fertilization and give rise to a complex organism. How does this happen? The Woodruff lab combines live-cell imaging, nano-rheology, and in vitro reconstiution to address how organelles assemble and disassemble following fertilization. The lab also focuses on protein quality control and stability in oocytes. With this knowledge, we can hopefully better understand how cell division errors arise during cellular ageing and contribute to cancer and fertility decline during advanced materal age.
- Biochemistry, cell biology, and synthetic biology
- CRISPR/Cas9 modification of embryos
- Material properties of organelles
- Oocyte quality and age-related fertility problems
- Regulation of centrosome size and number
- Organization and Function of Non-dynamic Biomolecular Condensates.
- Woodruff JB, Hyman AA, Boke E Trends Biochem. Sci. 2017 Dec
- Phosphatase PP2A and microtubule-mediated pulling forces disassemble centrosomes during mitotic exit.
- Enos SJ, Dressler M, Gomes BF, Hyman AA, Woodruff JB Biol Open 2017 Dec
- The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin.
- Woodruff JB, Ferreira Gomes B, Widlund PO, Mahamid J, Honigmann A, Hyman AA Cell 2017 Jun 169 6 1066-1077.e10
- Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro.
- Woodruff JB, Wueseke O, Viscardi V, Mahamid J, Ochoa SD, Bunkenborg J, Widlund PO, Pozniakovsky A, Zanin E, Bahmanyar S, Zinke A, Hong SH, Decker M, Baumeister W, Andersen JS, Oegema K, Hyman AA Science 2015 May 348 6236 808-12
- Polo-like kinase phosphorylation determines Caenorhabditis elegans centrosome size and density by biasing SPD-5 toward an assembly-competent conformation.
- Wueseke O, Zwicker D, Schwager A, Wong YL, Oegema K, Jülicher F, Hyman AA, Woodruff JB Biol Open 2016 Oct 5 10 1431-1440
- Method: In vitro analysis of pericentriolar material assembly.
- Woodruff JB, Hyman AA Methods Cell Biol. 2015 129 369-82
- The Caenorhabditis elegans pericentriolar material components SPD-2 and SPD-5 are monomeric in the cytoplasm before incorporation into the PCM matrix.
- Wueseke O, Bunkenborg J, Hein MY, Zinke A, Viscardi V, Woodruff JB, Oegema K, Mann M, Andersen JS, Hyman AA Mol. Biol. Cell 2014 Oct 25 19 2984-92
- Pericentriolar material structure and dynamics.
- Woodruff JB, Wueseke O, Hyman AA Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2014 Sep 369 1650
- Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle.
- Woodruff JB, Drubin DG, Barnes G Mol. Biol. Cell 2012 Jan 23 2 258-67
- Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8.
- Woodruff JB, Drubin DG, Barnes G J. Cell Biol. 2010 Nov 191 4 795-808
Honors & Awards
- CPRIT Recruitment Award
- EMBO Long-Term Postdoctoral Fellow
- Alexander von Humboldt Postdoctoral Fellow
- Weintraub Graduate Student Award nominee
- NSF Graduate Research Fellowship
- Dean's Honored Graduate, UT Austin