Tadamoto Isogai, Ph.D.

Since 2023, Tadamoto (Tada) Isogai has been appointed as an Assistant Professor at the Lyda Hill Department of Bioinformatics. Dr. Isogai received his PhD degrees from The Netherlands Cancer Institute in Amsterdam, The Netherlands.

Dr. Isogai combines high-resolution 3D single cell imaging and computer vision-assisted quantitation to study how the cytoskeletal architecture organizes morphology-driven signaling for cancer cell survival in vitro and in vivo. 

He further applies mathematical models inspired by financial economics to determine hierarchical information flow of genes embedded in complex molecular pathways with perturbation-free experimentation. Specific areas of interest include the regulation of actin-binding proteins in cell membrane protrusions. 

• Causal Inference in Molecular Pathways
• Cytoskeletal control of cell signaling
• Regulation of actin in pseudopod formation

Featured Publications

Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation.

Noh J, Isogai T, Chi J, Bhatt K, Danuser G, Cell Syst 2022 Jun 13 6 471-487.e8

Mechanical regulation of glycolysis via cytoskeleton architecture.

Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G, Nature 2020 02 578 7796 621-626

Discovery of functional interactions among actin regulators by analysis of image fluctuations in an unperturbed motile cell system.

Isogai T, Danuser G, Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2018 05 373 1747

Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2/3 complex.

Isogai T, van der Kammen R, Leyton-Puig D, Kedziora KM, Jalink K, Innocenti M, J. Cell. Sci. 2015 Oct 128 20 3796-810

Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes.

Jiang X, Isogai T, Chi J, Danuser G, PLoS Comput Biol 2022 Dec 18 12 e1009667

Particle retracking algorithm capable of quantifying large, local matrix deformation for traction force microscopy.

Haarman SE, Kim SY, Isogai T, Dean KM, Han SJ, PLoS One 2022 17 6 e0268614

Actin-Membrane Release Initiates Cell Protrusions.

Welf ES, Miles CE, Huh J, Sapoznik E, Chi J, Driscoll MK, Isogai T, Noh J, Weems AD, Pohlkamp T, Dean K, Fiolka R, Mogilner A, Danuser G, Dev Cell 2020 12 55 6 723-736.e8

Robust and automated detection of subcellular morphological motifs in 3D microscopy images.

Driscoll MK, Welf ES, Jamieson AR, Dean KM, Isogai T, Fiolka R, Danuser G, Nat. Methods 2019 Oct 16 10 1037-1044

Enhanced Dendritic Actin Network Formation in Extended Lamellipodia Drives Proliferation in Growth-Challenged Rac1^P29S Melanoma Cells.

Mohan AS, Dean KM, Isogai T, Kasitinon SY, Murali VS, Roudot P, Groisman A, Reed DK, Welf ES, Han SJ, Noh J, Danuser G, Dev. Cell 2019 05 49 3 444-460.e9

Profilin binding couples chloride intracellular channel protein CLIC4 to RhoA-mDia2 signaling and filopodium formation.

Argenzio E, Klarenbeek J, Kedziora KM, Nahidiazar L, Isogai T, Perrakis A, Jalink K, Moolenaar WH, Innocenti M, J. Biol. Chem. 2018 12 293 50 19161-19176

• Cecil H. and Ida Green Center for Systems Biology (2023)
• Lyda Hill Department of Bioinformatics (2023)