Nagesh Peddada, Ph.D. Assistant Professor School Medical School Department Center for Genetics of Host Defense | Immunology Graduate Programs Molecular Biophysics Biography Dr. Peddada obtained his undergraduate degree in Biotechnology from Madurai Kamaraj University and completed his Ph.D. in Protein Science and Engineering under the mentorship of Dr. Ashish at the CSIR-Institute of Microbial Technology in India. His doctoral research elucidated the structure-function relationship of human plasma gelsolin and explored its therapeutic potential. During his postdoctoral training in Jan Erzberger's laboratory at UT Southwestern Medical Center, Dr. Peddada specialized in the eukaryotic ribosome biogenesis pathway. He employed a novel hybrid structural approach, combining yeast genetics, single-particle cryo-electron microscopy (cryo-EM), and mass spectrometry to investigate the role of DEAD-box ATPases in rRNA remodeling events critical for ribosome assembly, with a particular focus on the transition of pre-60S ribosomes from the nucleolus to the nucleoplasm. Following his postdoctoral work, Dr. Peddada joined the Center for Genetics of Host Defense as an instructor with Nobel Laureate Dr. Bruce Beutler in 2021 and was promoted to Assistant Professor in 2024. In his current role, Dr. Peddada applies integrative methodologies, particularly single-particle cryo-EM, to elucidate the mechanisms underlying macromolecular complex assembly. His research investigates the roles of proteasome-activating factors, GTPases, and RNA/DNA binding proteins, which were identified through ENU mutagenesis Education Graduate School Madurai Kamaraj University (2009), Biotechnology Graduate School Jawaharlal Medical College (2014), Philosophy Research Interest Structural Biology, Immunology, Cell Biology Publications Featured Publications Visualizing the nucleating and capped states of f-actin by Ca2+-gelsolin: Saxs data based structures of binary and ternary complexes. Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish Int J Biol Macromol 2024 Aug 278 Pt 1 134556 Viable mutations of mouse midnolin suppress B cell malignancies. Zhong X, Peddada N, Moresco JJ, Wang J, Jiang Y, Rios JJ, Moresco EMY, Choi JH, Beutler B, J Exp Med 2024 Jun 221 6 The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis. Cruz VE, Weirich CS, Peddada N, Erzberger JP, Nat Commun 2024 Apr 15 1 3296 OVOL2 sustains postnatal thymic epithelial cell identity. Zhong X, Peddada N, Wang J, Moresco JJ, Zhan X, Shelton JM, SoRelle JA, Keller K, Lazaro DR, Moresco EMY, Choi JH, Beutler B, Nat Commun 2023 Nov 14 1 7786 Sequence-specific remodeling of a topologically complex RNP substrate by Spb4. Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP, Nat Struct Mol Biol 2022 Dec 29 12 1228-1238 Pregnancy-related hormones, progesterone and human chorionic gonadotrophin, upregulate expression of maternal plasma gelsolin. Garg R, Peddada N, Dolma K, Khatri N, Ashish Am J Physiol Regul Integr Comp Physiol 2018 04 314 4 R509-R522 Plasma Gelsolin Level in HIV-1-Infected Patients: An Indicator of Disease Severity. Sinha KK, Peddada N, Jha PK, Mishra A, Pandey K, Das VN, AshishDas P, AIDS Res Hum Retroviruses 2017 03 33 3 254-260 Plasma gelsolin levels decrease in diabetic state and increase upon treatment with F-actin depolymerizing versions of gelsolin. Khatri N, Sagar A, Peddada N, Choudhary V, Chopra BS, Garg V, Garg R, Ashish J Diabetes Res 2014 2014 152075 Global shapes of F-actin depolymerization-competent minimal gelsolins: insight into the role of g2-g3 linker in pH/Ca2+ insensitivity of the first half. Peddada N, Sagar A, Rathore YS, Choudhary V, Pattnaik UB, Khatri N, Garg R, Ashish J Biol Chem 2013 Sep 288 39 28266-82 Visualizing the elusive open shape of G-actin in solution by SAXS data analysis. Sagar A, Peddada N, Solanki Ak, Choudhary V, Garg R, Ashish Biochem Biophys Res Commun 2013 Jun 435 4 740-4 Results 1-10 of 14 1 2 Next Last Honors & Awards Excellence in Research PresentationIssued by Molecular Biophysics Graduate Program at 28th Annual Research symposium, UT Southwestern Medical Center (2019) Invited talk Genetic capture of nucleolar pre-60S ribosome structural intermediates during ribosome biogenesis2nd Biophysics Annual Retreat, USA, (2018) Senior Research FellowshipDepartment of Biotechnology, (DBT) (2011-2014) Junior Research Fellowship,Department of Biotechnology, (2009-2011) JNU-DBT-merit master's fellowshipMasters in BioTechnology (2007-2009)
