Biography

The Henne Lab is interested in how cells spatially organize their metabolism. This includes understanding how cells adapt to nutritional stress, and remodel their organelles to survive. Armed with this knowledge, we also hope to deeply understand molecular mechanisms that govern human physiology and disease.  In particular, we are currently focused on understanding how different organelles contact one another at so-called Membrane Contact Sites (MCSs), and how these MCSs are used as biological platforms for the exchange of nutrients and biological information. 

Recently, we characterized a new protein family (the PXA domain-containing family) that play critical roles in lipid metabolism and neurological disease (see Henne, JCB, 2015). In yeast, this protein is called Mdm1, and acts as a "molecular bridge" connecting the lysosome to the endoplasmic reticulum (ER).  Using a combination of cell biology and genetics, we have discovered that Mdm1 coordinates the production of lipid droplets (LDs) at a sub-region of the ER adjacent to the lysosome (Hariri, EMBO Reports, 2017). These LDs serve as nutrient reserviors, and are eventually delivered to lysosomes during starvation to promote survival. Our findings suggest a previously unappreciated level of spatial orgnaization to lipid droplet biology and cellular energetics. Indeed, this function is highly conserved in metazoans, and we find together with our clinical collaborator Dr. Phil Stanier (UCL, London) that human Mdm1 homolog SNX14 also functions in ER-lipid droplet crosstalk, the loss of which contributes to pediatric cerebellar ataxia (Bryant & Liu, HMG, 2018).

Background: Dr. Henne received his B.S. in Cellular and Molecular Biology from Texas Tech University in Lubbock, Texas, and then accepted a MRC Scholarship from the UK to pursue graduate studies at the MRC Laboratory of Molecular Biology at Cambridge University. As a student in the lab of Harvey McMahon, Ph.D., he studied how membrane sculpting BAR and F-BAR domain-containing proteins promote clathrin-mediated endocytosis. He characterized the F-BAR proteins FCHo1/2, and showed that they play crucial roles initiating clathrin vesicle biogenesis. Mike was awarded the Max Perutz Prize for his graduate work.

Following graduate school, Dr. Henne began a postdoctoral position in the laboratory of Scott Emr, Ph.D., at Cornell University as a Sam and Nancy Fleming Research Fellow. There, he continued to study endolysosomal trafficking, and how endosomes can be reshaped by the ESCRT (Endosomal Sorting Complexes Required for Transport) pathway. His work has focused on reconstituting and imaging ESCRT protein assemblies, and dissecting how they shape multi-vesicular endosomes. More recent projects involve global screens in yeast to identify novel proteins involved in endolysosomal trafficking.

Dr. Henne uses cell biology, biochemistry, structural biology, and genetics to understand the molecular mechanisms of membrane reshaping and trafficking. 

Research Interest

  • inter-organelle communication
  • lipid metabolism
  • membrane sculpting

Publications

Featured Publications LegendFeatured Publications

Classification of the human phox homology (PX) domains based on their phosphoinositide binding specificities.
Chandra M, Chin YK, Mas C, Feathers JR, Paul B, Datta S, Chen KE, Jia X, Yang Z, Norwood SJ, Mohanty B, Bugarcic A, Teasdale RD, Henne WM, Mobli M, Collins BM, Nat Commun 2019 Apr 10 1 1528
The assembly of lipid droplets and their roles in challenged cells.
Henne WM, Reese ML, Goodman JM EMBO J. 2018 May
Organelles in metabolism and stress responses.
Ferguson SM, Henne WM Mol. Biol. Cell 2018 Mar 29 6 691
Endoplasmic Reticulum-Vacuole Contact Sites "Bloom" With Stress-Induced Lipid Droplets.
Henne WM, Hariri H Contact (Thousand Oaks) 2018 Jan-Dec 1
larval feeding through insulin signaling and SLC5A11.
Ugrankar R, Theodoropoulos P, Akdemir F, Henne WM, Graff JM Commun Biol 2018 1 110

Honors & Awards

  • Searle Scholar
    (2016)
  • Sam & Nancy Fleming Research Fellowship
    (2011-2014)
  • The Max Perutz Prize
    awarded for Graduate work at MRC, Cambridge, UK (2009)