John Hulleman, Ph.D.
Assistant Professor

• Age-related macular degeneration
• Chemical biology
• Drug discovery
• Extracellular matrix proteins
• Inherited retinal dystrophies
• Ocular protein misfolding diseases
• Stress-responsive signaling

Featured Publications

Simultaneous Control of Endogenous and User-Defined Genetic Pathways Using Unique ecDHFR Pharmacological Chaperones

Ramadurgum P, Woodard DR, Daniel S, Douglas PM, Posner BA, Hulleman JD Cell Chem Biol 2020 27 May 21, 2020 1-13

Non-antibiotic Small-Molecule Regulation of DHFR-Based Destabilizing Domains In Vivo.

Peng H, Chau VQ, Phetsang W, Sebastian RM, Stone MRL, Datta S, Renwick M, Tamer YT, Toprak E, Koh AY, Blaskovich MAT, Hulleman JD, Mol Ther Methods Clin Dev 2019 Dec 15 27-39

A Destabilizing Domain Allows for Fast, Noninvasive, Conditional Control of Protein Abundance in the Mouse Eye - Implications for Ocular Gene Therapy.

Datta S, Renwick M, Chau VQ, Zhang F, Nettesheim ER, Lipinski DM, Hulleman JD Invest. Ophthalmol. Vis. Sci. 2018 Oct 59 12 4909-4920

An inducible form of Nrf2 confers enhanced protection against acute oxidative stresses in RPE cells.

Vu KT, Hulleman JD Exp. Eye Res. 2017 Aug 164 31-36

Conditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFkB Signaling in RPE Cells.

Vu KT, Zhang F, Hulleman JD Invest Ophthalmol Vis Sci 2017 58 10 4126-4137

Two C-terminal sequence variations determine differential neurotoxicity between human and mouse a-synuclein.

Landeck N, Strathearn KE, Ysselstein D, Buck K, Dutta S, Banerjee S, Lv Z, Hulleman JD, Hindupur J, Lin LK, Padalkar S, Stanciu LA, Lyubchenko YL, Kirik D, Rochet JC, Mol Neurodegener 2020 Sep 15 1 49

Delivery of Antisense Oligonucleotides to the Cornea.

Chau VQ, Hu J, Gong X, Hulleman JD, Ufret-Vincenty RL, Rigo F, Prakash TP, Corey DR, Mootha VV, Nucleic Acid Ther 2020 Mar

Expression of R345W-Fibulin-3 Induces Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells.

Zhou M, Weber SR, Zhao Y, Chen H, Barber AJ, Grillo SL, Wills CA, Wang HG, Hulleman JD, Sundstrom JM, Front Cell Dev Biol 2020 8 469

Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use

Ramadurgum P, Hulleman JD STAR Protocols 2020 1; September 18, 2020 100069 1-20

Protocol for In Vivo Evaluation and Use of Destabilizing Domains in the Eye, Liver, and Beyond

Ramadurgum P, Daniel S, Hulleman JD STAR Protocols 2020

 Featured Books

Alpha-Synuclein self-assembly in Parkinson's disease: Insights from biophysical analyses. In Protein Misfolding in Biology and Disease

Rochet JC and Hulleman JD (2009). Trivandrum, India, Research Signpost

• Bright Focus Foundation
  Macular Degeneration Research Award (2018-2020)
• National Institutes of Health
  R01EY027785 (2018-2023)
• National Institutes of Health
  R21EY028261 (2017-2019)
• Bright Focus Foundation
  Macular Degeneration Research Award (2016-2018)
• Karl Kirchgessner Foundation
  Vision Research Grant (2016-2019)
• Research to Prevent Blindness
  Career Development Award (2015-2019)