John Hulleman, Ph.D.
Assistant Professor

• Age-related macular degeneration
• Chemical biology
• Drug discovery
• Extracellular matrix proteins
• Inherited retinal dystrophies
• Ocular protein misfolding diseases
• Stress-responsive signaling

Featured Publications

Simultaneous Control of Endogenous and User-Defined Genetic Pathways Using Unique ecDHFR Pharmacological Chaperones

Ramadurgum P, Woodard DR, Daniel S, Douglas PM, Posner BA, Hulleman JD Cell Chem Biol 2020 27 May 21, 2020 1-13

Non-antibiotic Small-Molecule Regulation of DHFR-Based Destabilizing Domains In Vivo.

Peng H, Chau VQ, Phetsang W, Sebastian RM, Stone MRL, Datta S, Renwick M, Tamer YT, Toprak E, Koh AY, Blaskovich MAT, Hulleman JD, Mol Ther Methods Clin Dev 2019 Dec 15 27-39

A Destabilizing Domain Allows for Fast, Noninvasive, Conditional Control of Protein Abundance in the Mouse Eye - Implications for Ocular Gene Therapy.

Datta S, Renwick M, Chau VQ, Zhang F, Nettesheim ER, Lipinski DM, Hulleman JD Invest. Ophthalmol. Vis. Sci. 2018 Oct 59 12 4909-4920

An inducible form of Nrf2 confers enhanced protection against acute oxidative stresses in RPE cells.

Vu KT, Hulleman JD Exp. Eye Res. 2017 Aug 164 31-36

Conditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFkB Signaling in RPE Cells.

Vu KT, Zhang F, Hulleman JD Invest Ophthalmol Vis Sci 2017 58 10 4126-4137

Delivery of Antisense Oligonucleotides to the Cornea.

Chau VQ, Hu J, Gong X, Hulleman JD, Ufret-Vincenty RL, Rigo F, Prakash TP, Corey DR, Mootha VV, Nucleic Acid Ther 2020 Mar

Prospective Application of Activity-Based Proteomic Profiling in Vision Research-Potential Unique Insights into Ocular Protease Biology and Pathology.

Peng H, Hulleman JD, Int J Mol Sci 2019 Aug 20 16

Small Molecule-Based Inducible Gene Therapies for Retinal Degeneration.

Datta S, Peng H, Hulleman JD, Adv. Exp. Med. Biol. 2019 1185 65-69

Deducing the presence of proteins and proteoforms in quantitative proteomics.

Bamberger C, Martínez-Bartolomé S, Montgomery M, Pankow S, Hulleman JD, Kelly JW, Yates JR Nat Commun 2018 Jun 9 1 2320

High-Throughput Assay for Collagen Secretion Suggests an Unanticipated Role for Hsp90 in Collagen Production.

Wong MY, Doan ND, DiChiara AS, Papa LJ, Cheah JH, Soule CK, Watson N, Hulleman JD, Shoulders MD Biochemistry 2018 Apr

 Featured Books

Alpha-Synuclein self-assembly in Parkinson's disease: Insights from biophysical analyses. In Protein Misfolding in Biology and Disease

Rochet JC and Hulleman JD (2009). Trivandrum, India, Research Signpost

• Bright Focus Foundation
  Macular Degeneration Research Award (2018-2020)
• National Institutes of Health
  R01EY027785 (2018-2023)
• National Institutes of Health
  R21EY028261 (2017-2019)
• Bright Focus Foundation
  Macular Degeneration Research Award (2016-2018)
• Karl Kirchgessner Foundation
  Vision Research Grant (2016-2019)
• Research to Prevent Blindness
  Career Development Award (2015-2019)