John Hulleman, Ph.D.

• Age-related macular degeneration
• Chemical biology
• Drug discovery/high-throughput screening
• Extracellular matrix proteins
• Inherited retinal dystrophies
• Ocular protein misfolding diseases
• Stress-responsive signaling

Featured Publications

Development of a New DHFR-Based Destabilizing Domain with Enhanced Basal Turnover and Applicability in Mammalian Systems.

Nakahara E, Mullapudi V, Collier GE, Joachimiak LA, Hulleman JD, ACS Chem Biol 2022 Sep

A loss-of-function cysteine mutant in fibulin-3 (EFEMP1) forms aberrant extracellular disulfide-linked homodimers and alters extracellular matrix composition.

Woodard DR, Daniel S, Nakahara E, Abbas A, DiCesare SM, Collier GE, Hulleman JD, Hum Mutat 2022 Aug

Clinically-identified C-terminal mutations in fibulin-3 are prone to misfolding and destabilization.

Woodard DR, Nakahara E, Hulleman JD, Sci Rep 2021 Feb 11 1 2998

Fibulin-3 knockout mice demonstrate corneal dysfunction but maintain normal retinal integrity.

Daniel S, Renwick M, Chau VQ, Datta S, Maddineni P, Zode G, Wade EM, Robertson SP, Petroll WM, Hulleman JD, J. Mol. Med. 2020 Sep

Simultaneous Control of Endogenous and User-Defined Genetic Pathways Using Unique ecDHFR Pharmacological Chaperones

Ramadurgum P, Woodard DR, Daniel S, Douglas PM, Posner BA, Hulleman JD Cell Chem Biol 2020 27 May 21, 2020 1-13

A Destabilizing Domain Allows for Fast, Noninvasive, Conditional Control of Protein Abundance in the Mouse Eye - Implications for Ocular Gene Therapy.

Datta S, Renwick M, Chau VQ, Zhang F, Nettesheim ER, Lipinski DM, Hulleman JD Invest. Ophthalmol. Vis. Sci. 2018 Oct 59 12 4909-4920

SPECIFICATIONS OF THE ACMG/AMP VARIANT CURATION GUIDELINES FOR MYOCILIN: RECOMMENDATIONS FROM THE CLINGEN GLAUCOMA EXPERT PANEL.

Burdon KP, Graham P, Hadler J, Hulleman JD, Pasutto F, Boese EA, Craig JE, Fingert JH, Hewitt AW, Siggs OM, Whisenhunt K, Young TL, Mackey DA, Dubowsky A, Souzeau E, Hum Mutat 2022 Oct

Utility of the DHFR-based destabilizing domain across mouse models of retinal degeneration and aging.

Peng H, Ramadurgum P, Woodard DR, Daniel S, Nakahara E, Renwick M, Aredo B, Datta S, Chen B, Ufret-Vincenty R, Hulleman JD, iScience 2022 May 25 5 104206

A simple secretion assay for assessing new and existing myocilin variants.

Nakahara E, Hulleman JD, Curr Eye Res 2022 Feb 1-7

A trimethoprim derivative impedes antibiotic resistance evolution.

Manna MS, Tamer YT, Gaszek I, Poulides N, Ahmed A, Wang X, Toprak FCR, Woodard DR, Koh AY, Williams NS, Borek D, Atilgan AR, Hulleman JD, Atilgan C, Tambar U, Toprak E, Nat Commun 2021 May 12 1 2949

 Featured Books

Alpha-Synuclein self-assembly in Parkinson's disease: Insights from biophysical analyses. In Protein Misfolding in Biology and Disease

Rochet JC and Hulleman JD (2009). Trivandrum, India, Research Signpost

• Edward N. and Della L. Thome Memorial Foundation
  Awards Program in Age-Related Macular Degeneration Research (2023-2025)
• Fichtenbaum Charitable Trust
  Macular Degeneration Research Award (2022-2023)
• National Institutes of Health
  R21EY033522 (2022-2024)
• EyeFind Research Grant
  Human Donor Grant (2021-2022)
• Fichtenbaum Charitable Trust
  Macular Degeneration Research Award (2021-2022)
• Core Grant for Vision Research - Module Director
  P30EY030413 (2020-2025)
• Fichtenbaum Charitable Trust
  Macular Degeneration Research Award (2020-2021)
• Bright Focus Foundation
  Macular Degeneration Research Award (2018-2020)
• National Institutes of Health
  R01EY027785 (2018-2023)
• National Institutes of Health
  R21EY028261 (2017-2019)
• Bright Focus Foundation
  Macular Degeneration Research Award (2016-2018)
• Karl Kirchgessner Foundation
  Vision Research Grant (2016-2019)
• Research to Prevent Blindness
  Career Development Award (2015-2019)

• Investigator - Peter O'Donnell Jr. Brain Institute (2021)
• Adjunct Assistant Professor - University of North Texas Health Science Center (2016)