Biography

Download Curriculum Vitae

Universty of Texas Southwestern Medical Center Dallas, TX   01/2009 – Present  

- development of the original technique of mechanical immobilization of suspended isolated cultured skeletal myofibers and recording of the myofiber membrane potentials; 
- biophysical characterization of the skeletal muscle disease model mutant Na+ channels NaV1.4;
- development of the original microfluidic perfusion technique for high-performance cut-open oocyte voltage clamp recordings;
- single-channel current recordings from the endoplasmic reticulum ionic channels incorporated in the planar lipid bilayer membranes (Dept. Physiology);
- discovery of the membrane ionic channel-forming properties of the C-terminus of the Bax Inhibitor-1 protein (bilayer-incorporated; Dept. Physiology).

Research Fellow, Molecular Physiology and Therapeutics Branch

National Institutes of Health / NIDCR, Bethesda, MD    07/2010 – 09/2012

- biophysical and pharmacological characterization of the intracellular endosomal/lysosomal cationic channels TPC1 incorporated in lipid bilayers;
- development of the original electrical recording technique from lipid bilayer membranes, with enhanced cis and trans chamber perfusion capabilities;
- Ussing chamber electrical recordings from the epithelial cell monolayers on solid supports.

Research Assistant Professor, Pharmacology and Neuroscience

University of North Texas Health Science Center,  Fort Worth, TX   01/2004 – 12/2008 

- discovery of the protein-protein regulation of the intracellular Ca2+ release ryanodine receptors (RyR) by presenilin protein N-terminus;
- discovery of the modulation of the RyR by estrogen receptor beta;
- electrophysiological recordings of the ionic currents produced by the ionic channels and by a single nuclear pore in mammalian cell nuclei using a nuclear envelope-attached patch-pipette.

Research Associate, Department of Surgery

Yale University School of Medicine ,  New Haven, CT  11/2000 – 01/2004  

- discovery of a novel mechano-sensitive ion conductance in the baso-lateral membrane of the mammalian outer hair cells (OHC);
- discovery of the allosteric modulation of the OHC membrane motor protein prestin by multivalent anions and organic anions;
- recordings of the ultra-fast voltage-dependent motility and of a non-linear (“active”) membrane capacitance responses generated by the mammalian OHCs.

Instructor in Electrophysiology, Biology Department

University of Science and Technology - Lille 1Lille, France   04/1999 – 07/2000 

- discovery of the allosteric inhibition of the outwardly rectifying K+ channels by verapamil and by related phenylalkylamines in human prostate cancer LNCaP cell line;
- development of the equilibrium model of the ion channel double-inhibition by two channel blockers; 
- development of the custom image-analysis software for the semi-automatic tracing of the coordinates of the migrating cells in the long-term cell cultures.

Postdoctoral Fellow

Louis Pasteur University, Institute of Physiology and Biological Chemistry,  Strasbourg, France       03/1994 – 03/1999

- discovery of the low- and high-affinity activation of the AMPA(glut) receptors in spinal neurons;
- discovery of the multimodal GABA-A receptor activation by anxiolytic etifoxine in spinal neurons;
- patch-clamp recordings of the ACh, 5-HT3 and TRPV1 receptor-mediated currents in dorsal root ganglion (DRG) neurons; 
- patch-clamp study of the modulation of the K+ and Ca2+ channels in dorsal root ganglion neurons by the substance P ;
- development of the computer-operated mini-robotic version of the “concentration clamp” technique for ultra-fast application of the drugs to the isolated cells (15-20 compounds per cell).

Jr Research Scientist, Department of Biomedical Research

National Ukrainian Academy of Sciences, Institute of Bioorganic Chemistry and Petrochemistry,  Kiev, Ukraine   10/1987 – 02/1994

- patch-clamp study of the pharmacological properties of the structural molecule analogs of the glutamate/AMPA-receptor blocker APB;
- patch-clamp study of the kinetics properties of the AMPA(glut) receptors in the hippocampal neurons;
- development of the microfluidic pulsing jet for the high frequency (20 Hz) short term (5-10 ms) application of ligands to the isolated suspended patch-clamped neurons during electrical recordings.

Research Engineer

National Ukrainian Academy of Sciences, Bogomoletz Institute of Physiology,  Kiev, Ukraine   07/1984 – 09/1987

- U-tube patch-clamp study of the ion selectivity of the voltage-gated K+ channels in snail neurons; 
- U-tube patch-clamp study of the temperature-depended conformations in K+ channels; 
- biophysical modeling of the free-energy profile of the K+ channel voltage sensor transitions;
- development of the voltage/current clamp switch amplifier;
- development and fabrication of the custom precision micro-mechanics equipment for the electrophysiological patch-clamp recordings.

Education

Graduate School
Moscow Inst of Phys & Tech-Rus (1984), Physics Engineering
Graduate School
Moscow Inst of Phys & Tech-Rus (1994), Biological Sciences
Graduate School
(2017), Biology

Publications

Featured Publications LegendFeatured Publications

The C terminus of Bax inhibitor-1 forms a Ca2+-permeable channel pore.
Bultynck G, Kiviluoto S, Henke N, Ivanova H, Schneider L, Rybalchenko V, Luyten T, Nuyts K, De Borggraeve W, Bezprozvanny I, Parys JB, De Smedt H, Missiaen L, Methner A J. Biol. Chem. 2012 Jan 287 4 2544-57
Polycystin-2 expression and function in adult mouse lacrimal acinar cells.
Kaja S, Hilgenberg JD, Rybalchenko V, Medina-Ortiz WE, Gregg EV, Koulen P Invest. Ophthalmol. Vis. Sci. 2011 Jul 52 8 5605-11
The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca2+.
Rybalchenko V, Grillo MA, Gastinger MJ, Rybalchenko N, Payne AJ, Koulen P J. Recept. Signal Transduct. Res. 2009 Dec 29 6 326-41
On the temperature and tension dependence of the outer hair cell lateral membrane conductance GmetL and its relation to prestin.
Santos-Sacchi J, Rybalchenko V, Bai JP, Song L, Navaratnam D Pflugers Arch. 2006 Jun 452 3 283-9
Functional high- and low affinity agonist binding sites at native dorsal horn AMPA receptors.
Rybalchenko V, Schlichter R Neuroreport 1999 Jun 10 8 1803-6

Honors & Awards

  • Intramural Seed Research Grant
    University of North Texas Health Science Center at Fort Worth, TX (2006)
  • Postdoctoral Research Service Fellowship
    Eli Lilly Institute of France, Strasbourg, France (1996-1999)
  • Postdoctoral Research Fellowship
    Foundation for Medical Research of France (1995)
  • International Postdoctoral Trainee Fellowship
    National Ministry of Education, Research and Technology of France (1994)
  • “Engineering and Business in Canada”, Canada/Ukraine Government Exchange Program
    Ministry of Cultural and Scientific Affairs of Canada (1993)