Robert Orchard, Ph.D.

Download Curriculum Vitae

• CRISPR/Cas9 Screening
• Host-Pathogen Interactions
• Immunology
• Norovirus
• Virology

Featured Publications

Selective Polyprotein Processing Determines Norovirus Sensitivity to Trim7.

Sullender ME, Pierce LR, Annaswamy Srinivas M, Crockett SL, Dunlap BF, Rodgers R, Schriefer LA, Kennedy EA, Stewart BM, Doench JG, Baldridge MT, Orchard RC, J Virol 2022 Aug e0070722

Identification of anti-norovirus genes in human cells using genome-wide CRISPR activation screening.

Orchard RC, Sullender ME, Dunlap BF, Balce DR, Doench JG, Virgin HW J. Virol. 2018 Oct

Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor.

Nelson CA, Wilen CB, Dai YN, Orchard RC, Kim AS, Stegeman RA, Hsieh LL, Smith TJ, Virgin HW, Fremont DH Proc. Natl. Acad. Sci. U.S.A. 2018 09 115 39 E9201-E9210

Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.

Orchard RC, Wilen CB, Virgin HW Nat Microbiol 2018 Aug

Tropism for tuft cells determines immune promotion of norovirus pathogenesis.

Wilen CB, Lee S, Hsieh LL, Orchard RC, Desai C, Hykes BL, McAllaster MR, Balce DR, Feehley T, Brestoff JR, Hickey CA, Yokoyama CC, Wang YT, MacDuff DA, Kreamalmayer D, Howitt MR, Neil JA, Cadwell K, Allen PM, Handley SA, van Lookeren Campagne M, Baldridge MT, Virgin HW Science 2018 Apr 360 6385 204-208

Discovery of a proteinaceous cellular receptor for a norovirus.

Orchard RC, Wilen CB, Doench JG, Baldridge MT, McCune BT, Lee YC, Lee S, Pruett-Miller SM, Nelson CA, Fremont DH, Virgin HW Science 2016 08 353 6302 933-6

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.

Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE Nat. Biotechnol. 2016 Feb 34 2 184-191

Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.

Orchard RC, Kittisopikul M, Altschuler SJ, Wu LF, Süel GM, Alto NM Cell 2012 Feb 148 4 803-15

Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis.

Ortega-Gonzalez P, Taylor G, Jangra RK, Tenorio R, Fernandez de Castro I, Mainou BA, Orchard RC, Wilen CB, Brigleb PH, Sojati J, Chandran K, Sachse M, Risco C, Dermody TS, PLoS Pathog 2022 Mar 18 3 e1010322

UFMylation inhibits the proinflammatory capacity of interferon-?-activated macrophages.

Balce DR, Wang YT, McAllaster MR, Dunlap BF, Orvedahl A, Hykes BL, Droit L, Handley SA, Wilen CB, Doench JG, Orchard RC, Stallings CL, Virgin HW, Proc Natl Acad Sci U S A 2021 Jan 118 1

• UT Southwestern Medical Center (2008-2013)
• Washington University School of Medicine (2013-2018)