Asaithamby Aroumougame, Ph.D.

Assistant Professor

Department: Radiation Oncology

Graduate Programs: Cancer Biology

Biography

Download Curriculum Vitae

 Academic positions/employment

  1. Research Assistant Professor, Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Texas, 2009-Present.
  2. Instructor, Department of Radiation Oncology,UniversityofTexasSouthwesternMedicalCenterat Dallas, Texas, 2004-2008.
  3. Postdoctoral Fellow, Department of Life Sciences,LawrenceBerkeleyNational Laboratory, Berkeley, California, 2000-2004.
  4. Graduate student researcher,BanarasHinduUniversity, Uttar Pradesh, India, 1994-1999.

 

 FUNDINGS

  1. Functions of WRN in response to DNA double-strand breaks (From 04/01/09 to 03/31/14, 1R01CA134991-01A1, PI-David J. Chen, Co-I-Asaithamby A).

 

  1. Mechanisms of the Repair of HZE Induced DNA Double-strand Breaks in Human Cells, funded by NASA (10/1/07-09/30/12), NNAJ06ZSA001N, PI-David J. Chen, Co-I-Asaithamby A).

 

  1. Risks estimates and mechanisms of lung cancer pathogenesis after space radiation, funded by NASA NSCOR (01/01/2011-12/31/2016), NNX11AC54G, PI, John D. Minna, Co-I-Asaithamby A).

 

  1. Mechanisms of clustered DNA repair in human cells, submitted to NASA (10/01/2011-09/30/2015), PI-David J. Chen, Co-PI-Asaithamby A)

 

  1. Role of microRNAs in HZE particles induced carcinogenesis, submitted to NASA (10/01/2011-09/30/2015), PI-Michael D. Story, Co-I-Asaithamby A).

 Education

Degree

University

Year of passing

Marks (%)

Division

Specialization

B.Sc.

Pondicherry,India

1990

65

First

Life Science

M.Sc.

Pondicherry,India

1992

77

Distinction

Life Science

M.Phil.

Pondicherry,India

1993

78

Distinction

Endocrinology

NET

UGC-CSIR,India

1993

-

-

Life Science

Ph.D.

BanarasHindu,India

1999

-

-

Steroid hormones and Brain

Awards and Honors

  1. NASA young investigator award, 2007
  2. International Congress on Radiation Research Travel award, 2007.
  3. Radiation Research Society Scholar in Training Award, 2006.
  4. Distinction in M. Sc., 1992.
  5. University Second Rank in M. Sc., 1992.
  6. Distinction in M. Phil., 1994.

g.  Junior Research Fellowship, National Entrance Examination conducted by University Grants commission,India, 1993.

MEMBERSHIPS IN PROFESSIONAL SOCIETIES

  1. American Association for Advancement of Science, 2004-Present.
  2. Radiation Research Society (Associate Member), 2002-Present.

PUBLICATIONS

  1. Asaithamby, A*, B. Hu, and D.J. Chen* (2011). Unrepaired clustered DNA lesions induce chromosome breakage in human cells. Proc Natl Acad Sci U S A, 108(20): p. 8293-8 (*co-corresponding authors).

 

  1. Asaithamby A*, Hu B, Delgado O, Ding LH, Story MD, Minna JD, Shay JW, Chen DJ* (2011). Irreparable complex DNA double-strand breaks induce chromosome breakage in organotypic three-dimensional human lung epithelial cell culture. Nucleic Acids Res., 1;39(13):5474-5488 (*co-corresponding authors).
  2. Asaithamby, A* and D.J. Chen* (2011). Mechanism of cluster DNA damage repair in response to high-atomic number and energy particles radiation. Mutat Res, 711(1-2): p. 87-99, (*co-corresponding authors).

 

  1. Perry JJ*, Asaithamby A*, Barnebey A, Kiamanesch F, Chen DJ, Han S, Tainer JA, Yannone SM (2010). Identification of a coiled coil in werner syndrome protein that facilitates multimerization and promotes exonuclease processivity. J Biol Chem. 13;285(33):25699-707, (*Equal contribution).

 

  1. Kobayashi J, Okui M, Asaithamby A, Burma S, Chen BP, Tanimoto K, Matsuura S, Komatsu K and Chen DJ (2010). WRN participates in translesion synthesis pathway through interaction with NBS1. Mech Ageing Dev. 131(6):436-44.

 

  1. Mukherjee S, Coque L, Cao JL, Kumar J, Chakravarty S, Asaithamby A, Graham A, Gordon E, Enwright JF 3rd, DiLeone RJ, Birnbaum SG, Cooper DC, McClung CA (2010). Knockdown of Clock in the Ventral tegmental area through RNA interference results in a mixed state of mania and depression-like behavior. Biol. Psychiatry, 68(6):503-11.

 

  1. Asaithamby A and Chen DJ (2009).  Cellular Responses to DNA double-strand breaks after low-dose g-irradiation. Nucleic Acids Research, 37(12):3912-23.

 

  1. Fleisch MC, Chou YC, Cardiff RD, Asaithambi A, Shyamala G. (2009). Overexpression of progesterone receptor A isoform in mice leads to endometrial hyperproliferation, hyperplasia and atypia. Mol Hum Reprod, 15(4):241-9.

 

  1. Asaithamby A, Uematsu N., Chatterjee A, Story MD,Burma S and Chen DJ (2008). Repair of HZE-Particle-Induced DNA Double-Strand Breaks inNormal Human Fibroblasts. Radiat. Res. 169(4):437-46.

 

  1. Yano K, Yano K., Wang S, Uematsu N, Lee KJ, Asaithamby A, Weterings E and Chen DJ (2008). Ku recruits XLF to DNA double-strand breaks. EMBO reports, 9(1):91-6.

 

  1. Ramnarain D., Paulmurugan, Park S., Mickey B, Asaithamby A, Saha D., Kelliher MA., -- Habib A (2008). RIP1 links inflammatory and growth factor signaling pathways by regulating expression of EGFR. Cell death and differentiation, 15(2):344-53.

 

  1. Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA (2006). WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing. Nat Struct Mol Biol., 3(5):414-22.

 

  1. Chen BP, Chan DW, Kobayashi J, Burma S, Asaithamby A, Morotomi-Yano K, Botvinick E, Qin J, Chen DJ.(2005) Cell cycle dependence of DNA-dependent protein kinase phosphorylation in response to DNA double strand breaks. J Biol Chem., 280(15):1470 9-15.

 

  1. Lou Z, Chen BP, Asaithamby A, Minter-Dykhouse K, Chen DJ, Chen J. (2004).  MDC1 regulates DNA-PK autophosphorylation in response to DNA damage.  J Biol Chem., 279(45): 46359-62.

 

  1. Helguero LA, Viegas M, Asaithamby A, Shyamala G, Lanari C, Molinolo AA. (2003) Progesterone receptor expression in medroxyprogesterone acetate-induced murine mammary carcinomas and response to endocrine treatment. Breast Cancer Res Treat. 79(3): 379-90.

 

  1. Thakur M. K., Asaithambi A., Mukherjee S. 2000.  Amplification of exons 4 and 5 of androgen receptor gene by testosterone in the brain cortex of aged female mice.   Biogerontology, 1(4):329-34.

 

  1. Thakur M. K., Asaithambi A, Mukherjee S. (2000). Synthesis and Phosphorylation of Androgen Receptor of the mouse brain cortex and their Regulation by Sex Steroids during aging.  Molecular and Cellular Biochemistry, 203: 95-101.

 

  1. Asaithambi A., Mukherjee S. and Thakur M. K. (1999) Age-dependent degradation of Amyloid precursor protein in the post-mortem mice brain cortex.  Molecular Biology Reports 26 (3): 179-184.

 

  1. Mukherjee S., Asaithambi A. and Thakur M.K. (1999). Sex steroids modulate the synthesis and phosphorylation of specific proteins in the brain cortex of aging mice.  Mechanism Aging Development, 111 (1): 13-22.

 

  1. Thakur M. K., Asaithambi A. and Mukherjee S. (1999) Sex-specific alterations in chromatin conformation of the brain of aging mouse.  Molecular Biology Reports, 26: 239-247.

 

  1. Asaithambi A, Mukherjee S., Thakur M. K. (1997). Expression of 112kD estrogen receptor in mouse brain cortex and its auto regulation with age. Biochem. Biophys. Res. Commun. 231: 683-685.

 

  1. Mukherjee S., Asaithambi A., Thakur M. K. (1996). Androgen treatment protects mouse liver chromatin from cleavage by endogenous nucleases during aging. Molecular Biology Reports, 22:59-61.

 

9. INVITED TALKS AND PRESENTATIONS

  1. Asaithamby A, and Chen DJ (2011). Induction and Repair of Clustered DNA lesions repair in three-dimensional cell culture. Presented atDarmstadtUniversity of Technology, Radiation Biology and DNA Repair,Darmstadt,Germany, August 26,Darmstadt,Germany.

 

  1. Asaithamby A, and Chen DJ (2011). Visualization of Spatio-temporal Dynamics of Ionizing Radiation Induced Clustered DNA Lesions. Presented at the International Congress on Radiation Research, August 28-September 1Warsaw,Poland.

 

  1. Asaithamby A, and Chen DJ (2011). Unrepaired clustered DNA lesions induce chromosome breakage in human cells. Presented at the International Symposium for Radiation Research and Medical Physics, May 30-June 2,Shanghai,China.

 

  1. Asaithamby A, Hu B and Chen DJ (2010). Cellular Responses to DNA damages induced by high energy and high-Z particles irradiation. Presented at the NASA human research program Investigators’ workshop, February 3-5,Houston,Texas.
  2. Asaithamby A, Hu B and Chen DJ (2010). g-Radiation Induces Distinct DNA Damage Responses in a Human Lung Epithelial Cell Three-Dimensional Culture Model. Presented at Low-Dose Radiation Research Investigators’ workshop IX,Washington,DC, April 12-14.

 

  1. Asaithamby A, Hu B, Delgado D, Minna JD, Shay JW and Chen DJ (2010). DNA double-strand break repair is attenuated in three-dimensional human lung epithelial cell culture exposed to ionizing radiation. Presented at the 21st Annual NASA Space Radiation Investigators’ workshop, May 16-19, Port Jefferson, New York.

 

  1. Asaithamby A, Hu B and Chen DJ (2009). Cellular Responses to DNA damages induced by low- and high-linear energy transfer irradiation in monolayer and three-dimensional cell cultures. Invited presentation at the International Workshop on Radiation Biology and Radiation Protection, October 14-16,FudanUniversity,Shanghai,China.

 

  1. Asaithamby A and Chen DJ. (2009). Live cell imaging approach to directly monitor induction and repair of DNA damages generated by low- and high-linear energy transfer irradiation. Invited presented at the 55th Annual Radiation Research Society meeting, October 4-7,Savannah,Georgia.

 

  1. Asaithamby A and Chen DJ. (2009). Cellular responses to DNA double-strand breaks after low-dose g-irradiation. Presented at the Low-dose radiation research investigator’s workshop VIII, April 6-8,Bethesda,Maryland.

 

  1. Asaithamby A and Chen DJ (2009). Spatial and temporal characterization of DNA damage response to high and low-LET radiation in living cells.  Presented at the NASA human research program investigators’ workshop, February 2-4,League City,Texas.

 

  1. Asaithamby A, Oskar Gonzalez and Chen DJ. Visualization of DNA damage and repair following HZE particles radiation in living cells Presented at the NASA Human Research Program Investigator’s workshop, Feb 4-6, 2008,League City,TX.

 

  1. Asaithamby A, Oskar Gonzalez and Chen DJ (2008). Direct Visualization of Repair of DNA Double-Strand Breaks Induced by Low-Dose g-rays in Living Human Cell. Presented at the 19th Annual NASA Space Radiation Investigators’ Workshop, June 30-July 2,Philadelphia,Pennsylvania.

 

  1. Asaithamby A, Gonzales O and Chen DJ (2008). Visualization of low dose radiation induced DNA damage and repair in living cells. DOE low dose workshop, January 21-23,WashingtonDC.

 

  1. Asaithamby A, Gonzalez O and Chen DJ (2008). Visualization of HZE particles induced DNA damage and repair in living cells. NASA investigators workshop, February 4-6.League City,Texas.

 

  1. Asaithamby A, Uematsu N. and Chen D. J (2007). Visualization of HZE particles induced DNA double strand break repair in living cells. 18th NASA investigators workshop, July 13-15,Sanoma County,CA.

 

  1. Asaithamby A, Yannone S. M. and Chen D. J (2007). Phosphorylation of Werner Syndrome Protein by DNA-dependent protein kinase catalytic subunit is critical for its function in DNA repair.  13th International Congress on Radiation Research, July 8-12,San Francisco,CA.

 

  1. Asaithamby A, Yannone S. M., Qin J and Chen D. J (2006). Phosphorylation of Werner Syndrome Protein is critical for its function in DNA repair.  Radiation Research Society, November 5-8,Philadelphia,PA.

 

  1. Asaithamby A, Yannone S. M., Porteus M, Qin J and Chen D. J (2005). Phosphorylation of Werner Syndrome Protein is critical for its function in DNA repair.  Cold Spring Harbor Laboratory meeting on Telomere and Telomerase, May 4-8,Cold Spring Harbor,NY. 

 

  1. Asaithamby A, Yannone S. M., and Chen D. J.  (2004). Mapping of phosphorylation sites in the exonuclease domain of Werner syndrome protein. InLawrenceBerkeley National Laboratory, Life Sciences Division Postdoctoral Day, February 11,Berkeley,CA.

 

  1. Asaithamby A, Yannone S. M., Campisi J and Chen D. J.  (2003). Involvement of Werner syndrome protein in Non-homologous end joining (NHEJ) and DNA-PK dependent phosphorylation of its exonuclease domain. InLawrenceBerkeley National Laboratory, Life Sciences Division Postdoctoral Day, February 4,Berkeley,CA.

 

  1. Asaithamby A, Callow M and Shyamala G (2002).  Analysis of Gene expression pattern (by Microarray) in progesterone receptor (PR) A transgenic mice.  InLawrenceBerkeley National Laboratory, Life Sciences Division Postdoctoral Day, February 5,Berkeley,CA.

 

  1. Asaithamby A, Chou YC and Shyamala G (2001).  Uterine cell proliferation and progesterone receptor (PR) gene expression is altered in PR-A transgenic mice.  In: The endocrine Society’s 83rd Annual meeting, June 20-23,Denver,Colorado.

 

  1. Asaithambi A, Mukherjee S., & Thakur M. K. (1998).  Molecular regulation of androgen receptor in the brain cortex of aging mice.  EMBO workshop of nuclear hormone receptors, May 29-June 3,Nice,France.

 

UNDERGRADUATE AND GRADUATE RESEARCHERS TRAINED

  1. Katurah Spike, Berkeley Biotechnology Education, Inc. (BBEI), 2001.
  2. Luisa A Helguero, Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina, 2002.
  3. Peng Wei,UniversityofCaliforniaBerkeley, 2003.
  4. Foad Kiamanesh,UniversityofTexasDallas, 2005-2006.
  5. Michael Dodge, UT SouthwesternMedicalCenteratDallas, 2006.
  6. ShahedShakouri,UTSouthwestern Medical Center atDallas, 2006-2008.
  7. Oskar Gonzalez, UT SouthwesternMedicalCenteratDallas, 2007-2008.

 

POST-GRADUATE RESEARCHERS TRAINED

  1. Burong Hu,UTSouthwestern Medical Center, 2009-2010.
  2. Fengtao Su,UTSouthwestern Medical Center, 2011-Present
  1. Judge, 4th Annual Postdoctoral fellows’ Poster session, UT Southwestern Medical center,Dallas.

Reviewer For

  1. Aging Cell
  2. Cancer Research
  3. DNA repair
  4. Molecular and Cellular Biology
  5. Mutation Research
  6. Nucleic Acids research
  7. Proceeding ofNationalAcademyof Sciences
  8. Radiation Research
  9. Stem cells

Examiner for

1. Ph.D., thesis,Banaras Hindu University,India, 2005

2. Ph.D. thesis,Bhabha Atomic Research Center,India, 2010.

Education

Graduate School "pondicherry University, India" (), Reproductive Endocrinology
Graduate School Banaras Hindu University - ? (1998)

Research Interests

  • Biological Effects of Galactic Cosmic Rays on Astronauts
  • Fanconi Anemia and Cancer
  • How cells in different phases of the cell cycle recognise and response to stress?
  • Live cell imaging of sub-celluar DNA metablism
  • Replication Stress and Cancer
  • Werner Syndome and Cancer

Publications

Featured Publications Legend

Featured Publications

Evidence for formation of DNA repair centers and dose-response nonlinearity in human cells.

Neumaier T, Swenson J, Pham C, Polyzos A, Lo AT, Yang P, Dyball J, Asaithamby A, Chen DJ, Bissell MJ, Thalhammer S, Costes SV Proc. Natl. Acad. Sci. U.S.A. 2012 Jan 109 2 443-8

New insights into the roles of ATM and DNA-PKcs in the cellular response to oxidative stress.

Chen BP, Li M, Asaithamby A Cancer letters 2011 Dec

Unrepaired clustered DNA lesions induce chromosome breakage in human cells.

Asaithamby A, Hu B, Chen DJ Proc. Natl. Acad. Sci. U.S.A. 2011 May 108 20 8293-8

Unrepaired clustered DNA lesions induce chromosome breakage in human cells.

Asaithamby A, Hu B, Chen DJ PNAS 2011 20 108 8293-8

Irreparable complex DNA double-strand breaks induce chromosome breakage in organotypic three-dimensional human lung epithelial cell culture.

Asaithamby A, Hu B, Delgado O, Ding LH, Story MD, Minna JD, Shay JW, Chen DJ NAR 2011 13 39 5474-88

Identification of a coiled coil in werner syndrome protein that facilitates multimerization and promotes exonuclease processivity.

Perry JJ, Asaithamby A, Barnebey A, Kiamanesch F, Chen DJ, Han S, Tainer JA, Yannone SM JBC 2010 33 285 25699-707

Cellular responses to DNA double-strand breaks after low-dose gamma-irradiation.

Asaithamby A, Chen DJ NAR 2009 12 37 3912-23

WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing

Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA. Nat Struct Mol Biol 2006 5 13 414-22

A comparative study of ofloxacin and pivmecillinam in acute exacerbations of chronic bronchitis.

Egede F, Kristensen I Scand J Infect Dis Suppl 1990 68 46-9

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Professional Associations/Affiliations

  • American Association for the Advancement of Science
  • Radiation Research Society