Hani Suleiman, M.D., Ph.D. Assistant Professor School Medical School Department Internal Medicine | Biomedical Engineering Biography Hani Suleiman, M.D., Ph.D., is an Assistant Professor in the Department of Internal Medicine at UT Southwestern Medical Center and a member of its Division of Nephrology. He holds a secondary appointment in the Department of Biomedical Engineering. His research focuses on the dynamics of the actin cytoskeleton in kidney podocytes and the balance between Rho and Rac small GTPases in healthy and diseased kidneys. Originally from Syria, Dr. Suleiman earned his medical degree from Damascus University Faculty of Medicine. He completed his doctoral degree at the University of Regensburg in Germany and received postdoctoral training at Washington University School of Medicine in St. Louis. Dr. Suleiman joined the UT Southwestern faculty in 2024. Prior to UT Southwestern, he held the role of Assistant Professor of Nephrology at Washington University School of Medicine. Dr. Suleiman's approach to science involves the development and adaption of novel technologies to new biological questions. The cell of interest is the podocyte, an elaborate-looking cell that is important for kidney filtration function. Studying the podocytes provide an opportunity to study complex biological processes such as cell shape regulation, cell stability as well as the in-out and out-in signaling. His research interests span the areas of the actin cytoskeleton, isoactins, tropomyosins, and formins as well as questions related to the extracellular matrix and the interaction between the cells and their basement membranes. Dr. Suleiman's interests outside science includes his family, reading, swimming, and boxing. About the Suleiman Lab: Dr. Suleiman’s laboratory focuses on glomerular kidney diseases such as Minimal Change Disease (MCD), Focal Segmental Glomerulosclerosis (FSGS), Diabetic Nephropathy (DN), and Transplant Glomerulopathy (TG). The common feature in these diseases is the podocytopathy. It utilizes multiple modalities of super-resolution microscopic techniques, such as STORM, Airyscan, and N-Spark, as well as Light/Electron microscopic correlative approach. Dr. Suleiman’s laboratory recently developed a novel approach that can reveal the actin cytoskeleton in whole glomeruli, thus allowing them to interrogate the actin dynamics of podocytes in vivo, in both health and disease conditions. They also developed a new ex vivo system allowing them to study primary podocytes freshly isolated from isolated glomeruli and seeded on a defined substrate microprinted on a hydrogel system. Excitingly, this system provided, for the first time, an opportunity to observe phenomena in the primary podocytes that were never observed before. These novel techniques will help enable the design of new experiments and interpret old data differently. Research Interest Actin Cytoskeleton Diabetic Nephropathy Extracellular Matrix Fibrosis Formins Isoactins Podocyte Biology Transplant Glomerulopathy Tropomyosins Publications Quantitative assessment of glomerular basement membrane collagen IV α chains in paraffin sections from patients with focal segmental glomerulosclerosis and Alport gene variants Puapatanakul P, Isaranuwatchai S, Chanakul A, Surintrspanont J, Iampenkhae K, Kanjanabuch T, Suphapeetiporn K, Charu V, Suleiman HY, Praditpornsilpa K, Miner JH Kidney international 2024 May 105 1049-1057 Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures Langner E, Puapatanakul P, Pudlowski R, Alsabbagh DY, Miner JH, Horani A, Dutcher SK, Brody SL, Wang JT, Suleiman HY, Mahjoub MR Cytoskeleton 2024 An ex vivo culture model of kidney podocyte injury reveals mechanosensitive, synaptopodin-templating, sarcomere-like structures Jiang S, Alisafaei F, Huang YY, Hong Y, Peng X, Qu C, Puapatanakul P, Jain S, Miner JH, Genin GM, Suleiman HY Science Advances 2022 Sep 8 Three-Dimensional Visualization of the Podocyte Actin Network Using Integrated Membrane Extraction, Electron Microscopy, and Machine Learning Qu C, Roth R, Puapatanakul P, Loitman C, Hammad D, Genin GM, Miner JH, Suleiman HY Journal of the American Society of Nephrology 2022 Jan 33 155-173 Identification of an altered matrix signature in kidney aging and disease Randles MJ, Lausecker F, Kong Q, Suleiman H, Reid G, Kolatsi-Joannou M, Davenport B, Tian P, Falcone S, Potter P, van Agtmael T, Norman JT, Long DA, Humphries MJ, Miner JH, Lennon R Journal of the American Society of Nephrology 2021 Jul 32 1713-1732 Synaptopodin deficiency exacerbates kidney disease in a mouse model of Alport syndrome Ning L, Suleiman HY, Miner JH American Journal of Physiology - Renal Physiology 2021 Jul 321 F12-F25 Synaptopodin is dispensable for normal podocyte homeostasis but is protective in the context of acute podocyte injury Ning L, Suleiman HY, Miner JH Journal of the American Society of Nephrology 2020 Dec 31 2815-2832 Laminin-521 protein therapy for glomerular basement membrane and podocyte abnormalities in a model of pierson syndrome Lin MH, Miller JB, Kikkawa Y, Suleiman HY, Tryggvason K, Hodges BL, Miner JH Journal of the American Society of Nephrology 2018 May 29 1426-1436 Opposing roles of dendritic cell subsets in experimental GN Brähler S, Zinselmeyer BH, Raju S, Nitschke M, Suleiman H, Saunders BT, Johnson MW, Böhner AM, Viehmann SF, Theisen DJ, Kretzer NM, Briseño CG, Zaitsev K, Ornatsky O, Chang Q, Carrero JA, Kopp JB, Artyomov MN, Kurts C, Murphy KM, Miner JH, Shaw AS Journal of the American Society of Nephrology 2018 Jan 29 138-154 Ultrastructural Characterization of the Glomerulopathy in Alport Mice by Helium Ion Scanning Microscopy (HIM) Tsuji K, Suleiman H, Miner JH, Daley JM, Capen DE, Pǎunescu TG, Lu HA Scientific reports 2017 Dec 7 Results 1-10 of 24 1 2 3 Next Last Honors & Awards American Heart Association Award NephCure Foundation Award Professional Associations/Affiliations American Society of Cell Biology (ASCB) American Society of Nephrology (ASN) International Society of Glomerular Diseases (ISGD)