Don Gammon, Ph.D. Assistant Professor Endowed Title W.W. Caruth, Jr. Scholar in Biomedical Research School Medical School Department Microbiology Graduate Programs Molecular Microbiology Biography Dr. Gammon received his B.S. degree in Biological Sciences at the University of Windsor (Windsor, Canada) and went on to obtain his Ph.D. in Virology at the University of Alberta (Edmonton, Canada) in the laboratory of Dr. David Evans. While at the University of Alberta, Dr. Gammon’s research helped to elucidate the genetic and biochemical nature of poxvirus resistance to clinically-important acyclic nucleoside phosphonate drugs. In addition, he identified an unusual role for poxvirus DNA polymerase proofreading activity in catalyzing genetic recombination in virus-infected cells. His work also showed that poxviruses usurp host nucleotide biosynthetic machinery by forming novel “chimeric” ribonucleotide reductase enzymes that contain both viral and host proteins. This discovery led to Drs. Gammon and Evans obtaining a U.S. patent for the use of ribonucleotide reductase-deficient poxviruses in oncolytic virotherapy. Upon completion of his graduate research, Dr. Gammon pursued post-doctoral training in the laboratory of Nobel Laureate, Dr. Craig Mello, at the University of Massachusetts Medical School. In the Mello laboratory, he developed novel RNA virus-Lepidopteran (moth and butterfly) host systems to uncover both the immunity mechanisms used by eukaryotic organisms to restrict virus replication as well as the strategies viruses employ to counter such restrictions. These model systems arose from the observation that certain arboviruses undergo an abortive infection in Lepidopteran cells yet can replicate in these cells when host immunity is compromised during co-infection with other viruses, such as poxviruses. Using these model systems, his work has uncovered multiple conserved eukaryotic host factors that restrict arbovirus replication. These studies also identified a new family of highly conserved, microtubule-stabilizing virulence factors encoded by poxviruses that promote virus replication in Lepidopteran and vertebrate hosts. The Gammon laboratory uses a wide variety of cell culture-, virology-, molecular/cell biology, immunology, and biochemistry-related techniques to explore virus-host interplay. Using this multifaceted approach, along with newly-developed virus-host systems, Dr. Gammon’s team seeks to define the viral and host factors that ultimately determine virus host range and disease outcomes. Education Undergraduate University of Windsor (2004), Biological Sciences Graduate School University of Alberta (2010), Virology Research Interest Host-pathogen interactions Invertebrate host model systems RNA/DNA virus replication, immune evasion, and pathogenesis Viral manipulation of host cytoskeletal networks Publications Featured Publications Exploiting bacterial effector proteins to uncover evolutionarily conserved antiviral host machinery. Embry A, Baggett NS, Heisler DB, White A, de Jong MF, Kocsis BL, Tomchick DR, Alto NM, Gammon DB, PLoS Pathog 2024 May 20 5 e1012010 Poxvirus A51R Proteins Regulate Microtubule Stability and Antagonize a Cell-Intrinsic Antiviral Response Seo D., Oliveira, S.B., Rex, E.A, Ye, X., Rice, L.M., Guimaraes da Fonseca, F., and Gammon, D.B. Cell Rep 2024 43 3 1113882 FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins Rex, E.A., Seo, D., Chappidi, S., Pinkham, C., Oliveira, S.B., Embry, A., Heisler, D., Liu, Y., Munir, M., Luger, K., Alto, N.M., Guimaraes da Fonseca, F., Orchard, R., Hancks, D.H., and Gammon D.B. Nat Microbiol 2024 Poxvirus A51R Proteins Negatively Regulate Microtubule-Dependent Transport by Kinesin-1 Seo, D., Yue, Y., Yamazaki, S., Verhey, K.J., and Gammon, D.B. Int. J. Mol. Sci. 2024 14 25 7825 Gypsy moth genome provides insights into flight capability and virus-host interactions. Zhang J, Cong Q, Rex EA, Hallwachs W, Janzen DH, Grishin NV, Gammon DB Proc. Natl. Acad. Sci. U.S.A. 2019 Jan The Antiviral RNA Interference Response Provides Resistance to Lethal Arbovirus Infection and Vertical Transmission in Caenorhabditis elegans. Gammon DB, Ishidate T, Li L, Gu W, Silverman N, Mello CC Curr. Biol. 2017 Mar 27 6 795-806 A single vertebrate DNA virus protein disarms invertebrate immunity to RNA virus infection. Gammon DB, Duraffour S, Rozelle DK, Hehnly H, Sharma R, Sparks ME, West CC, Chen Y, Moresco JJ, Andrei G, Connor JH, Conte D, Gundersen-Rindal DE, Marshall WL, Yates JR, Silverman N, Mello CC Elife 2014 Jun 3 Exploiting Bacterial Effector Proteins to Uncover Evolutionarily Conserved Antiviral Host Machinery. Embry A, Baggett NS, Heisler DB, White A, de Jong MF, Kocsis BL, Tomchick DR, Alto NM, Gammon DB, bioRxiv 2024 Jan Abortive Infection of Animal Cells: What Goes Wrong Embry, A., and Gammon D.B. Annu. Rev. Virol. 2024 11 A FACT-ETS-1 Antiviral Response Pathway Restricts Viral Replication and is Countered by Poxvirus A51R Proteins. Rex EA, Seo D, Chappidi S, Pinkham C, Oliveira SB, Embry A, Heisler D, Liu Y, Luger K, Alto NM, da Fonseca FG, Orchard R, Hancks D, Gammon DB, bioRxiv 2023 Feb Results 1-10 of 25 1 2 3 Next Last Honors & Awards Alberta Innovates Health Solutions Fellowship Canadian Association for Graduate Studies Distinguished Dissertation Award Govenor General's Academic Gold Medal Izaak Walton Killam Scholar Natural Sciences and Engineering Research Council of Canada Fellowship Ralph Steinhauer Award of Distinction Professional Associations/Affiliations American Society for Virology (2005) Guest Editor, IJMS (2023) Guest Editor, Viruses (2020)
