Michael Reese, Ph.D.

Dr. Reese received a B.S. degree in Molecular Biophysics & Biochemistry from Yale University in 1998. While at Yale, he worked in the laboratory of Dr. Axel Brunger on structural studies of mammalian vesicular transport proteins (SNAPs and SNAREs). After Yale, Dr. Reese spent two years teaching high school science to gifted students with learning disabilities.

Dr. Reese received a Ph.D. in Biophysics in 2006 from the University of California, San Francisco, where he worked jointly in the laboratories of Dr. Frances Brodsky and Dr. Volker Doetsch on the structural basis for the activities of proteins involved in protein trafficking and neuronal signaling. During his postdoctoral work with John Boothroyd at Stanford University, Dr. Reese made the surprising discovery that a family of catalytically inactive kinases, or pseudokinases, are essential to Toxoplasma's ability to cause disease in mice.  Dr. Reese went on to demonstrate that these pseudokinases are allosteric inhibitors of the immune-related GTPases, which are critical for the control of a variety of intracellular pathogens.

In the fall of 2013, Dr. Reese joined the faculty in the Department of Pharmacology at UT Southwestern. His lab is focused on determining the many mechanisms by which the ubiquitous intracellular parasite, Toxoplasma gondii, co-opts the signaling networks of its host organisms. His laboratory combines techniques from multiple disciplines: from classical and molecular genetics and cell biology to biophysics and structural biology. This allows the examination of problems at many levels, from the atomic order structures of protein-protein complexes to the analysis of the signatures of evolutionary competition written in the genomes of the parasite and its hosts.

Undergraduate	Yale University (1998), Molecular Biophysics
Graduate School	University of California-San F (2006), Biophysics

• Biochemistry
• Biophysics
• Cell signaling, kinases, pseudokinases
• Host-pathogen interaction; co-evolution
• Immunology
• Structural biology

Featured Publications

A Toxoplasma gondii pseudokinase inhibits host IRG resistance proteins.

Fleckenstein MC, Reese ML, Könen-Waisman S, Boothroyd JC, Howard JC, Steinfeldt T PLoS Biol. 2012 10 7 e1001358

Polymorphic family of injected pseudokinases is paramount in Toxoplasma virulence.

Reese ML, Zeiner GM, Saeij JP, Boothroyd JC, Boyle JP Proc. Natl. Acad. Sci. U.S.A. 2011 Jun 108 23 9625-30

Impact of Regulated Secretion on Antiparasitic CD8 T Cell Responses.

Grover HS, Chu HH, Kelly FD, Yang SJ, Reese ML, Blanchard N, Gonzalez F, Chan SW, Boothroyd JC, Shastri N, Robey EA Cell Rep 2014 May

Expression of the essential kinase PfCDPK1 from Plasmodium falciparum in Toxoplasma gondii facilitates the discovery of novel antimalarial drugs.

Gaji RY, Checkley L, Reese M, Ferdig MT, Arrizabalaga G Antimicrob. Agents Chemother. 2014 Feb

A robust methodology to subclassify pseudokinases based on their nucleotide binding properties.

Murphy JM, Zhang Q, Young SN, Reese ML, Bailey FP, Eyers PA, Ungureanu D, Hammarén H, Silvennoinen O, Varghese LN, Chen K, Tripaydonis A, Jura N, Fukuda K, Qin J, Nimchuk Z, Mudgett MB, Elowe S, Gee CL, Liu L, Daly RJ, Manning G, Babon JJ, Lucet IS Biochem. J. 2013 Oct

Virulence without catalysis: how can a pseudokinase affect host cell signaling?

Reese ML, Boyle JP Trends Parasitol. 2012 Feb 28 2 53-7

A conserved non-canonical motif in the pseudoactive site of the ROP5 pseudokinase domain mediates its effect on Toxoplasma virulence.

Reese ML, Boothroyd JC J. Biol. Chem. 2011 Aug 286 33 29366-75

Chemical genetic screen identifies Toxoplasma DJ-1 as a regulator of parasite secretion, attachment, and invasion.

Hall CI, Reese ML, Weerapana E, Child MA, Bowyer PW, Albrow VE, Haraldsen JD, Phillips MR, Sandoval ED, Ward GE, Cravatt BF, Boothroyd JC, Bogyo M Proc. Natl. Acad. Sci. U.S.A. 2011 Jun 108 26 10568-73

Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements.

Kodama Y, Reese ML, Shimba N, Ono K, Kanamori E, Dötsch V, Noguchi S, Fukunishi Y, Suzuki E, Shimada I, Takahashi H J. Struct. Biol. 2011 Jun 174 3 434-42

Toxoplasma rhoptry protein 16 (ROP16) subverts host function by direct tyrosine phosphorylation of STAT6.

Ong YC, Reese ML, Boothroyd JC J. Biol. Chem. 2010 Sep 285 37 28731-40

Featured Books

Interactions Between Toxoplasma Effectors and Host Immune Respones. In Toxoplasma gondii. 2nd edition. K. Kim and L. Weiss (eds.)

Butcher BA, Reese ML, Boothroyd JC, Denkers EY. (2013). London, Academic Press

• NIAID K22 Research Scholar Development Award
  Career transition grant (2011)
• American Cancer Society Postdoctoral Research Fellowship
  (2009-2011)
• National Science Foundation Graduate Research Fellow
  (2003-2006)