An important manifestation of the phenotype is the regulation of metabolism, from nucleotide synthesis to intermediary metabolism. Since regulation cumulates in modification of pathway fluxes, measurement of the latter is an important aspect of understanding metabolism. The more informative means of estimating flux involves the use of tracers, and the combination of the stable isotope 13C and 13C NMR spectroscopy has proved particularly valuable. Due to the complexity of intermediary metabolism and the data yield available from labeled substrates (particularly when more than one distinctively-labeled substrate is provided), mathematical modeling is required. Various models have been developed and provided as software for use in the scientific community. Some of the work carried out has also involved the complementary technique mass spectrometry (in particular, tandem mass spectrometry). These methods can be applied to a variety of experimental models, from isolated organelles to the whole body. The primary focus of the work has been metabolism of heart, liver and (most recently) brain.
|Undergraduate||Oxford University - England (1981), Biochemistry|
|Graduate School||Oxford University - England (1985)|
- 13C NMR spectroscopy
- In Vivo and Ex Vivo measurement of pathway fluxes
- Mathematical models of metabolism
- Myocardial Metabolism
- Non-invasive studies of intermediary metabolism
- Metabolism of [U-(13) C]glucose in human brain tumors in vivo.
- Maher EA, Marin-Valencia I, Bachoo RM, Mashimo T, Raisanen J, Hatanpaa KJ, Jindal A, Jeffrey FM, Choi C, Madden C, Mathews D, Pascual JM, Mickey BE, Malloy CR, Deberardinis RJ NMR in biomedicine 2012 Mar
- Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR.
- Merritt ME, Harrison C, Storey C, Jeffrey FM, Sherry AD, Malloy CR Proc. Natl. Acad. Sci. U.S.A. 2007 Dec 104 50 19773-7
- Tricarboxylic acid cycle inhibition by Li+ in the human neuroblastoma SH-SY5Y cell line: a 13C NMR isotopomer analysis.
- Fonseca CP, Jones JG, Carvalho RA, Jeffrey FM, Montezinho LP, Geraldes CF, Castro MM Neurochem. Int. 2005 Nov 47 6 385-93
- Effect of murine strain on metabolic pathways of glucose production after brief or prolonged fasting.
- Burgess SC, Jeffrey FM, Storey C, Milde A, Hausler N, Merritt ME, Mulder H, Holm C, Sherry AD, Malloy CR Am. J. Physiol. Endocrinol. Metab. 2005 Jul 289 1 E53-61
- A (13)C isotopomer kinetic analysis of cardiac metabolism: influence of altered cytosolic redox and [Ca(2+)](o).
- Carvalho RA, Rodrigues TB, Zhao P, Jeffrey FM, Malloy CR, Sherry AD Am. J. Physiol. Heart Circ. Physiol. 2004 Aug 287 2 H889-95
- Analytical solutions for (13)C isotopomer analysis of complex metabolic conditions: substrate oxidation, multiple pyruvate cycles, and gluconeogenesis.
- Sherry AD, Jeffrey FM, Malloy CR Metab. Eng. 2004 Jan 6 1 12-24
- 13C isotopomer analysis of glutamate by tandem mass spectrometry.
- Jeffrey FM, Roach JS, Storey CJ, Sherry AD, Malloy CR Anal. Biochem. 2002 Jan 300 2 192-205
- TCA cycle kinetics in the rat heart by analysis of (13)C isotopomers using indirect (1)H.
- Carvalho RA, Zhao P, Wiegers CB, Jeffrey FM, Malloy CR, Sherry AD Am. J. Physiol. Heart Circ. Physiol. 2001 Sep 281 3 H1413-21
- NMR indirect detection of glutamate to measure citric acid cycle flux in the isolated perfused mouse heart.
- Burgess SC, Babcock EE, Jeffrey FM, Sherry AD, Malloy CR FEBS Lett. 2001 Sep 505 1 163-7
- Use of a single (13)C NMR resonance of glutamate for measuring oxygen consumption in tissue.
- Jeffrey FM, Reshetov A, Storey CJ, Carvalho RA, Sherry AD, Malloy CR Am. J. Physiol. 1999 Dec 277 6 Pt 1 E1111-21
Honors & Awards
- British Heart Foundation Studentship
- University of Oxford Entrance Scholarship